Polymerase-chain-reaction-based detection of individual polyhydroxyalkanoate synthase phaC1 and phaC2 genes

被引：12
|
作者：
Solaiman, DKY [1 ]
机构：
[1] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 04期
关键词：
Comamonas; P; corrugata; PCR; PHA synthase; poly(hydroxyalkanoates); Pseudomonas oleovorans;
D O I：
10.1023/A:1014017609866
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A semi-nested PCR method for the specific and individual amplification of type II phaC1 and phaC2 subgenomic fragments containing the catalytically important lipase box-like sequence and the phosphopantetheine binding site was developed. Direct sequencing of these DeltaphaC1 and DeltaphaC2 fragments is possible, thus greatly facilitating the characterization of these genes. The method was used to show that three strains of Pseudomonas oleovorans harbor different pha loci, while five strains of P. corrugata contain identical DeltaphaC1 and DeltaphaC2 subgenomic fragments.
引用
收藏
页码：245 / 250
页数：6
相关论文
共 50 条
  • [21] MUTANT OLIGONUCLEOTIDE EXTENSION AMPLIFICATION - A NONLABELING POLYMERASE-CHAIN-REACTION-BASED ASSAY FOR THE DETECTION OF POINT MUTATIONS
    EFREMOV, DG
    DIMOVSKI, AJ
    JANKOVIC, L
    EFREMOV, GD
    ACTA HAEMATOLOGICA, 1991, 85 (02) : 66 - 70
  • [22] Development and application of a heterologous internal standard for polymerase-chain-reaction-based detection of Campylobacter jejuni and Campylobacter coli in foods
    Straub, JA
    Hertel, C
    Mäde, D
    Hammes, WP
    EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 1999, 209 (3-4) : 180 - 184
  • [23] Development and application of a heterologous internal standard for polymerase-chain-reaction-based detection of Campylobacter jejuni and Campylobacter coli in foods
    J. A. Straub
    C. Hertel
    D. Mäde
    W. P. Hammes
    European Food Research and Technology, 1999, 209 : 180 - 184
  • [24] Detection of Pseudomonas aeruginosa by a triplex polymerase chain reaction assay based on lasI/R and gyrB genes
    Aghamollaei, Hosseine
    Moghaddam, Mehrdad M.
    Kooshki, Hamid
    Heiat, Mohammad
    Mirnejad, Reza
    Barzi, Nastaran S.
    JOURNAL OF INFECTION AND PUBLIC HEALTH, 2015, 8 (04) : 314 - 322
  • [25] POLYMERASE CHAIN REACTION-BASED METHODS FOR THE DETECTION OF MUTATIONS IN ONCOGENES AND TUMOR-SUPPRESSOR GENES
    LODA, M
    HUMAN PATHOLOGY, 1994, 25 (06) : 564 - 571
  • [26] Rapid multiplex polymerase chain reaction for detection of mcr-1 to mcr-5 genes
    Lescat, Mathilde
    Poirel, Laurent
    Nordmann, Patrice
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2018, 92 (04) : 267 - 269
  • [27] Detection and Differentiation of CAV-1 and CAV-2 by Polymerase Chain Reaction
    R.L. Hu
    G. Huang
    W. Qiu
    Z.H. Zhong
    X.Z. Xia
    Z. Yin
    Veterinary Research Communications, 2001, 25 : 77 - 84
  • [28] Detection and differentiation of CAV-1 and CAV-2 by polymerase chain reaction
    Hu, RL
    Huang, G
    Qiu, W
    Zhong, ZH
    Xia, XZ
    Yin, Z
    VETERINARY RESEARCH COMMUNICATIONS, 2001, 25 (01) : 77 - 84
  • [29] Polymerase chain reaction for the detection of flaA-1 genes of oral spirochaetes in human advanced periodontal pockets
    Sato, T
    Kuramitsu, HK
    ARCHIVES OF ORAL BIOLOGY, 2000, 45 (10) : 921 - 925
  • [30] Polymerase-chain-reaction-based detection of fetal rhesus D and Y-chromosome-specific DNA in the whole blood of pregnant women during different trimesters of pregnancy
    Al-Yatama, M. K.
    Mustafa, A. S.
    Al-Kandari, F. M.
    Khaja, N.
    Zohra, K.
    Monem, R. A.
    Abraham, S.
    MEDICAL PRINCIPLES AND PRACTICE, 2007, 16 (05) : 327 - 332
12345