Polymerase-chain-reaction-based detection of individual polyhydroxyalkanoate synthase phaC1 and phaC2 genes

被引：12
|
作者：
Solaiman, DKY [1 ]
机构：
[1] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 04期
关键词：
Comamonas; P; corrugata; PCR; PHA synthase; poly(hydroxyalkanoates); Pseudomonas oleovorans;
D O I：
10.1023/A:1014017609866
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A semi-nested PCR method for the specific and individual amplification of type II phaC1 and phaC2 subgenomic fragments containing the catalytically important lipase box-like sequence and the phosphopantetheine binding site was developed. Direct sequencing of these DeltaphaC1 and DeltaphaC2 fragments is possible, thus greatly facilitating the characterization of these genes. The method was used to show that three strains of Pseudomonas oleovorans harbor different pha loci, while five strains of P. corrugata contain identical DeltaphaC1 and DeltaphaC2 subgenomic fragments.
引用
收藏
页码：245 / 250
页数：6
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