An interleukin-6 ZnO/SiO2/Si surface acoustic wave biosensor

被引：71
|
作者：
Krishnamoorthy, Soumya [1 ]
Iliadis, Agis A. [1 ,2 ]
Bei, Thaleia [3 ]
Chrousos, George P. [4 ,5 ]
机构：
[1] Univ Maryland, Dept Elect & Comp Engn, College Pk, MD 20742 USA
[2] Univ Aegean, Dept Informat & Commun Syst Engn, Aegean, Greece
[3] NICHHD, Sect Endocrinol & Genet, Dev Endocrinol Branch, NIH, Bethesda, MD 20892 USA
[4] NIH, Sect Pediat Endocrinol, Reprod & Mol Biol Branch, Bethesda, MD 20892 USA
[5] Univ Athens, Sch Med, Dept Pediat 1, GR-11527 Athens, Greece
来源：
BIOSENSORS & BIOELECTRONICS | 2008年 / 24卷 / 02期
基金：
美国国家科学基金会;
关键词：
interleukin-6; ZnO; SAW biosensor; protein immobilization; SiO2/Si;
D O I：
10.1016/j.bios.2008.04.011
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
A novel high sensitivity ZnO/SiO2/Si Love mode surface acoustic wave (SAW) biosensor for the detection of interleukin-6 (IL-6), is reported. The biosensors operating at 747.7 MHz and 1.586 GHz were functionalized by immobilizing the monoclonal IL-6 antibody onto the ZnO biosensor surface both through direct surface adsorption and through covalent binding on gluteraldehyde. The morphology of the IL-6 antibody-protein complex was studied using scanning electron microscopy (SEM), and the mass of the IL-6 protein immobilized on the surface was measured from the frequency shift of the SAW resonator biosensor. The biosensor was shown to have extended linearity, which was observed to improve with higher sensor frequency and for IL-6 immobilization through the monoclonal antibody. Preliminary results of biosensor measurements of low levels of IL-6 in normal human serum are reported. The biosensor can be fully integrated with CMOS Si chips and developed as a portable real time detection system for the interleukin family of proteins in human serum. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：313 / 318
页数：6
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