Functionalization of Si/SiO2 substrates with homooligonucleotides for a DNA biosensor

被引：0
|
作者：
Cloarec, JP [1 ]
Martin, JR [1 ]
Polychronakos, C [1 ]
Lawrence, I [1 ]
Lawrence, MF [1 ]
Souteyrand, E [1 ]
机构：
[1] Ecole Cent Lyon, IFOS, PCI, F-69131 Ecully, France
来源：
EUROSENSORS XII, VOLS 1 AND 2 | 1998年
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D O I：
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中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Si/SiO2 substrates are functionnalized with single stranded homooligonucleotides in order to implement a DNA biosensor based on field effect measurement. Two methods of immobilization of homooligonucleotides on Si/SiO2 substrates, using an aminosilane and a glycidoxysilane, are described. Both methods are characterized by radiolabelling and electrochemical impedance measurements on Electrolyte/Dielectric/Semiconductor structures. The protocol using glycidoxysilane allows a strong and high density immobilization of oligonucleotides.
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页码：793 / 796
页数：4
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