Functionalization of Si/SiO2 substrates with homooligonucleotides for a DNA biosensor

被引：0

作者：

Cloarec, JP ^{[1
]}

Martin, JR ^{[1
]}

Polychronakos, C ^{[1
]}

Lawrence, I ^{[1
]}

Lawrence, MF ^{[1
]}

Souteyrand, E ^{[1
]}

机构：

[1] Ecole Cent Lyon, IFOS, PCI, F-69131 Ecully, France

来源：

EUROSENSORS XII, VOLS 1 AND 2 | 1998年

关键词：

D O I：

暂无

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Si/SiO2 substrates are functionnalized with single stranded homooligonucleotides in order to implement a DNA biosensor based on field effect measurement. Two methods of immobilization of homooligonucleotides on Si/SiO2 substrates, using an aminosilane and a glycidoxysilane, are described. Both methods are characterized by radiolabelling and electrochemical impedance measurements on Electrolyte/Dielectric/Semiconductor structures. The protocol using glycidoxysilane allows a strong and high density immobilization of oligonucleotides.

引用

页码：793 / 796

页数：4

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