DNA-based immunization against hepatitis B surface antigen carrying preS epitopes

被引：4

作者：

Hui, JY ^{[1
]}

Li, GD ^{[1
]}

Kong, YY ^{[1
]}

Wang, Y ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 1999年 / 44卷 / 07期

关键词：

preS epitopes; hepatitis B surface antigen (HBsAg); DNA-based immunization; antibody;

D O I：

10.1007/BF03182722

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Three plasmid expression vectors containing modified hepatitis B surface antigen (HBsAg) carrying preS epitopes were constructed. Transient expression after in vitro transfection in COS-M6 cells showed that under the transcriptional control of the human cytomegalovirus (CMV) immediate early promoter, fusion genes expressed the modified HBV envelope proteins which were efficiently secreted into culture medium and presented HBsAg, preS1 and preS2 antigenicity. DNA-based immunization with these plasmids carrying preS sequences induced anti-HBs antibody in BALB/c mice. The titers of anti-HBs antibody were higher than those appeared in mice immunized with plasmid carrying S gene only. DNA injection with plasmids containing preS1 sequences elicited also high titers of anti-preS1 antibody. Moreover, the anti-preS1 antibodies were found to appear earlier than anti-HBs antibodies.

引用

页码：620 / 623

页数：4

共 50 条

[41] DNA vaccines for prophylactic or therapeutic immunization against hepatitis B
Michel, ML
Loirat, D
INTERVIROLOGY, 2001, 44 (2-3) : 78 - 87
[42] PROTECTIVE IMMUNIZATION AGAINST HEPATITIS-B WITH AN INTERNAL ANTIGEN OF THE VIRUS
MURRAY, K
BRUCE, SA
WINGFIELD, P
VANEERD, P
DEREUS, A
SCHELLEKENS, H
JOURNAL OF MEDICAL VIROLOGY, 1987, 23 (02) : 101 - 107
[43] Hepatitis B immunization cause detectable hepatitis B surface antigen in hemodialysis patients.
Ly, D
Saab, S
Martin, P
Brezina, M
Gitnick, G
Yee, HF
HEPATOLOGY, 2000, 32 (04) : 382A - 382A
[44] DNA-based immunization of mice against HBV by intramuscular injection requires free DNA
McCluskie, MJ
Davis, HL
VACCINES 97 - MOLECULAR APPROACHES TO THE CONTROL OF INFECTIOUS DISEASES, 1997, : 125 - 130
[45] DNA-mediated immunization to hepatitis B surface antigen: Longevity of primary response and effect of boost
Davis, HL
Mancini, M
Michel, ML
Whalen, RG
VACCINE, 1996, 14 (09) : 910 - 915
[46] Monoclonal antibodies to various epitopes of hepatitis B surface antigen inhibit hepatitis B virus infection
Shirazi, Forough Golsaz
Mohammadi, Hamed
Amiri, Mohammad Mehdi
Singethan, Katrin
Xia, Yuchen
Bayat, Ali Ahmad
Bahadori, Motahareh
Rabbani, Hodjatallah
Jeddi-Tehrani, Mahmood
Protzer, Ulrike
Shokri, Fazel
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2014, 29 (05) : 1083 - 1091
[47] Hepatitis B virus DNA and hepatitis B surface antigen levels in chronic hepatitis B
Fung, James
Lai, Ching-Lung
Yuen, Man-Fung
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY, 2010, 8 (06) : 717 - 726
[48] Detection of hepatitis B surface antigen, hepatitis B core antigen, and hepatitis B virus DNA in parotid tissues
Chen, Lizhang
Liu, Fuqiang
Fan, Xuegong
Gao, Jianming
Chen, Nengzhi
Wong, Tom
Wu, Jun
Wen, Shi Wu
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2009, 13 (01) : 20 - 23
[49] Localization of hepatitis B surface antigen epitopes present on variants and specifically recognised by anti-hepatitis B surface antigen monoclonal antibodies
Jolivet-Reynaud, C
Lésenéchal, M
O'Donnell, B
Becquart, L
Foussadier, A
Forge, F
Battail-Poirot, N
Lacoux, X
Carman, W
Jolivet, M
JOURNAL OF MEDICAL VIROLOGY, 2001, 65 (02) : 241 - 249
[50] Cellular and humoral immune response to hepatitis B virus structural proteins in mice after DNA-based immunization
Geissler, M
Tokushige, K
Chante, CC
Zurawski, VR
Wands, JR
GASTROENTEROLOGY, 1997, 112 (04) : 1307 - 1320

← 1 2 3 4 5 →