DNA-based immunization against hepatitis B surface antigen carrying preS epitopes

被引：4
|
作者：
Hui, JY [1 ]
Li, GD [1 ]
Kong, YY [1 ]
Wang, Y [1 ]
机构：
[1] Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 1999年 / 44卷 / 07期
关键词：
preS epitopes; hepatitis B surface antigen (HBsAg); DNA-based immunization; antibody;
D O I：
10.1007/BF03182722
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Three plasmid expression vectors containing modified hepatitis B surface antigen (HBsAg) carrying preS epitopes were constructed. Transient expression after in vitro transfection in COS-M6 cells showed that under the transcriptional control of the human cytomegalovirus (CMV) immediate early promoter, fusion genes expressed the modified HBV envelope proteins which were efficiently secreted into culture medium and presented HBsAg, preS1 and preS2 antigenicity. DNA-based immunization with these plasmids carrying preS sequences induced anti-HBs antibody in BALB/c mice. The titers of anti-HBs antibody were higher than those appeared in mice immunized with plasmid carrying S gene only. DNA injection with plasmids containing preS1 sequences elicited also high titers of anti-preS1 antibody. Moreover, the anti-preS1 antibodies were found to appear earlier than anti-HBs antibodies.
引用
收藏
页码：620 / 623
页数：4
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