Expression and characterization of catA(1) (catechol 1,2-dioxygenase I-1) of Acinetobacter lwoffii K24 in Escherichia coli

被引：0

作者：

Kim, SI ^{[1
]}

Kweon, SM ^{[1
]}

Kim, S ^{[1
]}

Ha, KS ^{[1
]}

机构：

[1] KOREA BASIC SCI INST, BIOMOL RES GRP, TAEJON 305333, SOUTH KOREA

来源：

JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1997年 / 30卷 / 05期

关键词：

catechol; 12-dioxygenase; expression; purification;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Catechol 1.2-dioxygenase I-1 (CD I-1) gene of Acinetobacter lwoffii K24, catA(1) was expressed in Escherichia coli and was partially purified by using a MonoQ column. Expressed CD I-1 had the same molecular weight as purified CD I-1 from A. lwoffii K24 on SDS-PAGE. Expressed CD I-1 was also identified by Western blotting and peptide sequencing of N-terminal and internal regions. When compared with purified CD I-1 of A. lwoffii K24. expressed CD I-1 had similar substrate specificities and the effects of compounds on enzyme activity. N-terminal amino acid sequence of CD I-1 expressed in E. coli was the same as that of purified CD I-1. suggesting that CD I-1 may be under the same posttranslational processing in E. coli and A. lwoffii K24.

引用

页码：342 / 345

页数：4

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