Expression and characterization of catA(1) (catechol 1,2-dioxygenase I-1) of Acinetobacter lwoffii K24 in Escherichia coli

Catechol 1.2-dioxygenase I-1 (CD I-1) gene of Acinetobacter lwoffii K24, catA(1) was expressed in Escherichia coli and was partially purified by using a MonoQ column. Expressed CD I-1 had the same molecular weight as purified CD I-1 from A. lwoffii K24 on SDS-PAGE. Expressed CD I-1 was also identified by Western blotting and peptide sequencing of N-terminal and internal regions. When compared with purified CD I-1 of A. lwoffii K24. expressed CD I-1 had similar substrate specificities and the effects of compounds on enzyme activity. N-terminal amino acid sequence of CD I-1 expressed in E. coli was the same as that of purified CD I-1. suggesting that CD I-1 may be under the same posttranslational processing in E. coli and A. lwoffii K24.