ENZYMATIC ACTIVITY OF CATECHOL 1,2-DIOXYGENASE AND CATECHOL 2,3-DIOXYGENASE PRODUCED BY Gordonia polyisoprenivorans

被引：40
|
作者：
Silva, Andrea Scaramal [1 ]
de Oliveira Camargo, Flavio Anastacio [1 ]
Andreazza, Robson [1 ,4 ]
Seminoti Jacques, Rodrigo Josemar [2 ]
Baldoni, Daiana Bortoluzzi [2 ]
Bento, Fatima M. [3 ]
机构：
[1] Univ Fed Rio Grande do Sul, Dept Ciencia Solo, BR-91541000 Porto Alegre, RS, Brazil
[2] Univ Fed Santa Maria, Dept Ciencia Solo, BR-97105900 Santa Maria, RS, Brazil
[3] Univ Fed Rio Grande do Sul, Dept Microbiol, BR-91541000 Porto Alegre, RS, Brazil
[4] Univ Fed Pelotas, Ctr Engn, BR-96010280 Pelotas, RS, Brazil
来源：
QUIMICA NOVA | 2012年 / 35卷 / 08期
关键词：
anthracene; enzyme activity; enzyme immobilization; BIODEGRADATION; STRAIN; DEGRADATION; SLUDGE; SOIL; OIL; REMEDIATION; HYDROCARBON; BIOREACTOR; ANTHRACENE;
D O I：
10.1590/S0100-40422012000800018
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
This study aimed to evaluate the environmental conditions for enzyme activity of catechol 1,2-dioxygenase (C1,2O) and catechol 2,3-dioxygenase (C2,3O) produced by Gordonia polyisoprenivorans in cell-free and immobilized extracts. The optimum conditions of pH, temperature, time course and effect of ions for enzyme activity were determined. Peak activity of C1,2O occurred at pH 8.0. The isolate exhibited the highest activity of C2,3O at pH 7.0 and 8.0 for the cell-free extract and immobilized extract, respectively. This isolate exhibited important characteristics such as broad range of pH, temperature and time course for enzyme activity.
引用
收藏
页码：1587 / 1592
页数：6
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