A new FRET-based ratiometric fluorescent probe for the detection of SO2 derivatives in mitochondria of living cells

被引：23
|
作者：
Wang, Wei [1 ]
Li, Na [2 ]
Liu, Jin-Ting [1 ]
Miao, Jun-Ying [2 ]
Zhao, Bao-Xiang [1 ]
Lin, Zhao-Min [3 ]
机构：
[1] Shandong Univ, Sch Chem & Chem Engn, Inst Organ Chem, Jinan 250100, Peoples R China
[2] Shandong Univ, Sch Life Sci, Inst Dev Biol, Qingdao 266237, Peoples R China
[3] Shandong Univ, Inst Med Sci, Hosp 2, Jinan 250033, Peoples R China
来源：
DYES AND PIGMENTS | 2020年 / 181卷
基金：
中国国家自然科学基金;
关键词：
Fluorescence; FRET; Ratiometric; Mitochondria-targeted; HSO3-/SO32-; SULFUR-DIOXIDE DERIVATIVES; BORON DIPYRROMETHENE; SELECTIVE DETECTION; HYPOCHLOROUS ACID; BISULFITE; RHODAMINE; CYSTEINE; SULFITE; HYDRAZIDE; PLATFORM;
D O I：
10.1016/j.dyepig.2020.108639
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
We developed a new mitochondria-targeted ratiometric fluorescent probe (named PPA) for SO2 derivatives (HSO3-/SO32-) with a large (pseudo) Stokes shift (209 nm) based on FRET platform in which water-soluble (E)-4-(4-(dimethylamino)styryl)-1-alkylpyridin-1-ium was chosen as acceptor moiety and phenothiazine oxide as donor moiety. Probe PPA showed specific response toward HSO3-/SO32- over H2S, GSH, Cys and Hcy. The recognition group underwent a nucleophilic addition reaction with HSO3-/SO(3)(2- )to give rise to clear dual-emission signal changes in enhanced blue channel and subdued red channel under a single wavelength excitation. Moreover, probe PPA was successfully used for the detection of endogenous HSO3-/SO32- and bioimaging of mitochondria-targeted in living cells.
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页数：6
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