Microplates for Crystal Growth and in situ Data Collection at a Synchrotron Beamline

被引:6
|
作者
Liang, Miao [1 ,2 ]
Wang, Zhijun [1 ,3 ]
Wu, Hai [1 ,2 ]
Yu, Li [1 ,2 ]
Sun, Bo [1 ,3 ]
Zhou, Huan [1 ,3 ]
Yu, Feng [1 ,3 ]
Wang, Qisheng [1 ,3 ]
He, Jianhua [1 ,4 ]
机构
[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China
[4] Wuhan Univ, Inst Adv Studies, Wuhan 430072, Peoples R China
来源
CRYSTALS | 2020年 / 10卷 / 09期
关键词
microcrystals; microplate; grid scanning; in situ data collection; X-RAY-DIFFRACTION; PROTEIN CRYSTALLIZATION; CRYSTALLOGRAPHY; CHIP; SOFTWARE; PLATFORM; DROPLET;
D O I
10.3390/cryst10090798
中图分类号
O7 [晶体学];
学科分类号
0702 ; 070205 ; 0703 ; 080501 ;
摘要
An efficient data collection method is important for microcrystals, because microcrystals are sensitive to radiation damage. Moreover, microcrystals are difficult to harvest and locate owing to refraction effects from the surface of the liquid drop or optically invisible, owing to their small size. Collecting X-ray diffraction data directly from the crystallization devices to completely eliminate the crystal harvesting step is of particular interest. To address these needs, novel microplates combining crystal growth and data collection have been designed for efficient in situ data collection and fully tested at Shanghai Synchrotron Radiation Facility (SSRF) crystallography beamlines. The design of the novel microplates fully adapts the advantage of in situ technology. Thin Kapton membranes were selected to seal the microplate for crystal growth, the crystallization plates can support hanging drop and setting drop vapor diffusion crystallization experiments. Then, the microplate was fixed on a magnetic base and mounted on the goniometer head for in situ data collection. Automatic grid scanning was applied for crystal location with a Blu-Ice data collection system and then in situ data collection was performed. The microcrystals of lysozyme were selected as the testing samples for diffraction data collection using the novel microplates. The results show that this method can achieve comparable data quality to that of the traditional method using the nylon loop. In addition, our method can efficiently and diversely perform data acquisition experiments, and be especially suitable for solving structures of multiple crystals at room temperature or cryogenic temperature.
引用
收藏
页码:1 / 15
页数:15
相关论文
共 50 条
  • [31] Launch of the I13-2 data beamline at the Diamond Light Source synchrotron
    Bodey, A. J.
    Rau, C.
    [J]. X-RAY MICROSCOPY CONFERENCE 2016 (XRM 2016), 2017, 849
  • [32] Launch of the I13-2 data beamline at the Diamond Light Source synchrotron
    Bodey, A.J.
    Rau, C.
    [J]. Journal of Physics: Conference Series, 2017, 849 (01):
  • [33] Use of Zero Order Synchrotron Radiation for In-situ Cleaning of Beamline Optics: Results of Trial Experiments
    Yadav, P. K.
    Gupta, R. K.
    Modi, M. H.
    [J]. DAE SOLID STATE PHYSICS SYMPOSIUM 2018, 2019, 2115
  • [34] In situ crystal data-collection and ligand-screening system at SPring-8
    Okumura, Hideo
    Sakai, Naoki
    Murakami, Hironori
    Mizuno, Nobuhiro
    Nakamura, Yuki
    Ueno, Go
    Masunaga, Takuya
    Kawamura, Takashi
    Baba, Seiki
    Hasegawa, Kazuya
    Yamamoto, Masaki
    Kumasaka, Takashi
    [J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2022, 78 : 241 - 251
  • [35] Automated Data Collection Based on RoboDiff at the ESRF Beamline MASSIF-1
    Nurizzo, Didier
    Bowler, Matthew W.
    Guichard, Nicolas
    McSweeney, Sean
    Theveneau, Pascal
    Guijarro, Matias
    Svensson, Olof
    Mueller-Dieckmann, Christoph
    Leonard, Gordon
    [J]. PROCEEDINGS OF THE 12TH INTERNATIONAL CONFERENCE ON SYNCHROTRON RADIATION INSTRUMENTATION (SRI2015), 2016, 1741
  • [36] Remote Synchrotron Data Collection and Structure Determination Pipeline.
    Gilski, Miroslaw
    [J]. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 2009, 65 : S116 - S117
  • [37] Structure refinement against synchrotron Laue data: Strategies for data collection and reduction
    Yang, XJ
    Ren, Z
    Moffat, K
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 : 367 - 377
  • [38] OPTIMISING SYNCHROTRON FACILITIES FOR DATA COLLECTION IN PROTEIN CRYSTALLOGRAPHY.
    Nave, Colin
    [J]. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 1996, 52 : C18 - C18
  • [39] Efficient use of synchrotron radiation for macromolecular diffraction data collection
    Dauter, Z
    [J]. PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 2005, 89 (02): : 153 - 172
  • [40] Single pulse data collection with an X-ray chopper at in situ room temperature Laue crystallography beamline BL03HB
    Yu, Li
    Wang, Zhijun
    Pan, Cheng
    Zhang, Weizhe
    Sun, Bo
    Wang, Sisheng
    Wang, Qisheng
    Wen, Wen
    Gao, Xingyu
    He, Jianhua
    [J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 2024, 1069