Prenyl Alcohol Production by Expression of Exogenous Isopentenyl Diphosphate Isomerase and Farnesyl Diphosphate Synthase Genes in Escherichia coli

被引：14
|
作者：
Ohto, Chikara [2 ]
Muramatsu, Masayoshi [2 ]
Obata, Shusei [2 ]
Sakuradani, Eiji [1 ]
Shimizu, Sakayu [1 ]
机构：
[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan
[2] Toyota Motor Co Ltd, Biol Res Lab, Toyota 4718572, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 01期
关键词：
farnesol; geranylgeraniol; lipoate; pyruvate; terpenoid; ISOPRENOID BIOSYNTHESIS; IDENTIFICATION;
D O I：
10.1271/bbb.80446
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Isopentenyl diphosphate isomerase (idi) and farnesyl diphosphate synthase (ispA) genes were overexpressed in Escherichia coli. The resulting transformant showed 6.8-fold higher production of farnesol (389 mu g/l). In a similar manner, overexpression of idi and mutated ispA led to high production of geranylgeraniol (128 mu g/1).
引用
收藏
页码：186 / 188
页数：3
相关论文
共 50 条
  • [31] Effects of Exogenous Elicitors on Triterpenoids Accumulation and Expression of Farnesyl Diphosphate Synthase Gene in Inonotus obliquus
    Pei Lin
    Zheng-Fei Yan
    Chang-Tian Li
    Biotechnology and Bioprocess Engineering, 2020, 25 : 580 - 588
  • [32] Effects of Exogenous Elicitors on Triterpenoids Accumulation and Expression of Farnesyl Diphosphate Synthase Gene inInonotus obliquus
    Lin, Pei
    Yan, Zheng-Fei
    Li, Chang-Tian
    BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, 2020, 25 (04) : 580 - 588
  • [33] Characterization of the Escherichia coli undecaprenyl diphosphate synthase
    Kadrmas, JL
    Poulter, CD
    FASEB JOURNAL, 2001, 15 (04): : A192 - A192
  • [34] Identification of multi-gene families encoding isopentenyl diphosphate isomerase in plants by heterologous complementation in Escherichia coli
    Cunningham, FX
    Gantt, E
    PLANT AND CELL PHYSIOLOGY, 2000, 41 (01) : 119 - 123
  • [35] Molecular cloning and tissue expression of an insect farnesyl diphosphate synthase
    Castillo-Gracia, M
    Couillaud, F
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 262 (02): : 365 - 370
  • [36] Deregulated expression and activity of Farnesyl Diphosphate Synthase (FDPS) in Glioblastoma
    Abate, Mario
    Laezza, Chiara
    Pisanti, Simona
    Torelli, Giovanni
    Seneca, Vincenzo
    Catapano, Giuseppe
    Montella, Francesco
    Ranieri, Roberta
    Notarnicola, Maria
    Gazzerro, Patrizia
    Bifulco, Maurizio
    Ciaglia, Elena
    SCIENTIFIC REPORTS, 2017, 7
  • [37] Regulation of farnesyl diphosphate synthase gene expression by fatty acids
    Le Jossic-Corcos, C
    Zaghini, I
    Gonthier, C
    Logette, E
    Bournot, P
    PEROXISOMAL DISORDERS AND REGULATION OF GENES, 2003, 544 : 333 - 334
  • [38] Deregulated expression and activity of Farnesyl Diphosphate Synthase (FDPS) in Glioblastoma
    Mario Abate
    Chiara Laezza
    Simona Pisanti
    Giovanni Torelli
    Vincenzo Seneca
    Giuseppe Catapano
    Francesco Montella
    Roberta Ranieri
    Maria Notarnicola
    Patrizia Gazzerro
    Maurizio Bifulco
    Elena Ciaglia
    Scientific Reports, 7
  • [39] Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production
    Kim, SW
    Keasling, JD
    BIOTECHNOLOGY AND BIOENGINEERING, 2001, 72 (04) : 408 - 415
  • [40] Role of isopentenyl-diphosphate isomerase in heterologous cyanobacterial (Synechocystis) isoprene production
    Chaves, Julie E.
    Romero, Paloma Rueda
    Kirst, Henning
    Melis, Anastasios
    PHOTOSYNTHESIS RESEARCH, 2016, 130 (1-3) : 517 - 527
12345