Prenyl Alcohol Production by Expression of Exogenous Isopentenyl Diphosphate Isomerase and Farnesyl Diphosphate Synthase Genes in Escherichia coli

被引：14

作者：

Ohto, Chikara ^{[2
]}

Muramatsu, Masayoshi ^{[2
]}

Obata, Shusei ^{[2
]}

Sakuradani, Eiji ^{[1
]}

Shimizu, Sakayu ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan

[2] Toyota Motor Co Ltd, Biol Res Lab, Toyota 4718572, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 01期

关键词：

farnesol; geranylgeraniol; lipoate; pyruvate; terpenoid; ISOPRENOID BIOSYNTHESIS; IDENTIFICATION;

D O I：

10.1271/bbb.80446

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isopentenyl diphosphate isomerase (idi) and farnesyl diphosphate synthase (ispA) genes were overexpressed in Escherichia coli. The resulting transformant showed 6.8-fold higher production of farnesol (389 mu g/l). In a similar manner, overexpression of idi and mutated ispA led to high production of geranylgeraniol (128 mu g/1).

引用

页码：186 / 188

页数：3

共 50 条

[21] Substrate specificity of thermostable farnesyl diphosphate synthase with respect to 4-alkyl group homologs of isopentenyl diphosphate
Nagaki, M
Yamamoto, H
Takahashi, A
Maki, Y
Ishibashi, J
Nishino, T
Koyama, T
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2002, 17 (02) : 81 - 89
[22] FARNESYL-DIPHOSPHATE SYNTHASE - CATALYSIS OF AN INTRAMOLECULAR PRENYL TRANSFER WITH BISUBSTRATE ANALOGS
DAVISSON, VJ
NEAL, TR
POULTER, CD
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (04) : 1235 - 1245
[23] PURIFICATION AND CHARACTERIZATION OF RECOMBINANT HUMAN FARNESYL DIPHOSPHATE SYNTHASE EXPRESSED IN ESCHERICHIA-COLI
DING, VDH
SHEARES, BT
BERGSTROM, JD
PONPIPOM, MM
PEREZ, LB
POULTER, CD
BIOCHEMICAL JOURNAL, 1991, 275 : 61 - 65
[24] Structure Conservation and Differential Expression of Farnesyl Diphosphate Synthase Genes in Euphorbiaceous Plants
Guo, Dong
Li, Hui-Liang
Peng, Shi-Qing
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2015, 16 (09) : 22402 - 22414
[25] Escherichia coli open reading frame 696 Is idi, a nonessential gene encoding isopentenyl diphosphate isomerase
Hahn, FM
Hurlburt, AP
Poulter, CD
JOURNAL OF BACTERIOLOGY, 1999, 181 (15) : 4499 - 4504
[26] Expression and characterization of Escherichia coli undecaprenyl-diphosphate synthase
Kadrmas, JL
Poulter, CD
FASEB JOURNAL, 1999, 13 (07): : A1380 - A1380
[27] Elicitor-mediated induction of anthraquinone biosynthesis and regulation of isopentenyl diphosphate isomerase and farnesyl diphosphate synthase activities in cell suspension cultures of Cinchona robusta How.
Ana C. Ramos-Valdivia
Robert van der Heijden
Robert Verpoorte
Planta, 1997, 203 : 155 - 161
[28] Cloning, expression and characterization of a dipteran farnesyl diphosphate synthase
Sen, Stephanie E.
Trobaugh, Corey
Beliveau, Catherine
Richard, Thenesha
Cusson, Michel
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2007, 37 (11) : 1198 - 1206
[29] Elicitor-mediated induction of anthraquinone biosynthesis and regulation of isopentenyl diphosphate isomerase and farnesyl diphosphate synthase activities in cell suspension cultures of Cinchona robusta How.
RamosValdivia, AC
vanderHeijden, R
Verpoorte, R
PLANTA, 1997, 203 (02) : 155 - 161
[30] Culture conditions affect the category and production of ubiquinones in a recombinant escherichia coli with an exogenous decaprenyl diphosphate synthase
Li, Jiazhou
Zhang, Yuanyuan
Xie, Mingquan
Luo, Xiaochun
Luo, X., 1600, Maxwell Science Publications, 74, Kenelm Road,, B10, 9AJ, Birmingham, Small Heath, United Kingdom (05): : 1186 - 1191

← 1 2 3 4 5 →