Prenyl Alcohol Production by Expression of Exogenous Isopentenyl Diphosphate Isomerase and Farnesyl Diphosphate Synthase Genes in Escherichia coli

被引：14
|
作者：
Ohto, Chikara [2 ]
Muramatsu, Masayoshi [2 ]
Obata, Shusei [2 ]
Sakuradani, Eiji [1 ]
Shimizu, Sakayu [1 ]
机构：
[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan
[2] Toyota Motor Co Ltd, Biol Res Lab, Toyota 4718572, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 01期
关键词：
farnesol; geranylgeraniol; lipoate; pyruvate; terpenoid; ISOPRENOID BIOSYNTHESIS; IDENTIFICATION;
D O I：
10.1271/bbb.80446
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Isopentenyl diphosphate isomerase (idi) and farnesyl diphosphate synthase (ispA) genes were overexpressed in Escherichia coli. The resulting transformant showed 6.8-fold higher production of farnesol (389 mu g/l). In a similar manner, overexpression of idi and mutated ispA led to high production of geranylgeraniol (128 mu g/1).
引用
收藏
页码：186 / 188
页数：3
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