Characterization of a thermostable endo-β-1,4-D-galactanase from the hyperthermophile Thermotoga maritima

被引：15

作者：

Yang, H

Ichinose, H

Yoshida, M

Nakajima, M

Kobayashi, H

Kaneko, S

机构：

[1] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

[2] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 02期

关键词：

glycoside hydrolase family 53; pectin; endo-beta 1,4-D-galactanase; Thermotoga maritima;

D O I：

10.1271/bbb.70.538

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A putative endo-beta-1,4-D-galactanase gene of Thermotoga maritima was cloned and overexpressed in Escherichia coli. The recombinant enzyme hydrolyzed pectic galactans and produced D-galactose, beta-1,4-D-galactobiose, beta-1,4-D-galactotriose, and beta-1,4-D-galactotetraose. The enzyme displayed optimum activity at 90 degrees C and pH 7.0. It was slowly inactivated above pH 8.0 and below pH 5.0 and stable at temperatures up to 80 degrees C.

引用

页码：538 / 541

页数：4

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