Characterization of a thermostable endo-β-1,4-D-galactanase from the hyperthermophile Thermotoga maritima

被引：15
|
作者：
Yang, H
Ichinose, H
Yoshida, M
Nakajima, M
Kobayashi, H
Kaneko, S
机构：
[1] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan
[2] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058572, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2006年 / 70卷 / 02期
关键词：
glycoside hydrolase family 53; pectin; endo-beta 1,4-D-galactanase; Thermotoga maritima;
D O I：
10.1271/bbb.70.538
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A putative endo-beta-1,4-D-galactanase gene of Thermotoga maritima was cloned and overexpressed in Escherichia coli. The recombinant enzyme hydrolyzed pectic galactans and produced D-galactose, beta-1,4-D-galactobiose, beta-1,4-D-galactotriose, and beta-1,4-D-galactotetraose. The enzyme displayed optimum activity at 90 degrees C and pH 7.0. It was slowly inactivated above pH 8.0 and below pH 5.0 and stable at temperatures up to 80 degrees C.
引用
收藏
页码：538 / 541
页数：4
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