Molecular cloning and anti-fungal effect of endo-β-1,3-glucanase from Thermotoga maritima

被引：10
|
作者：
Woo, Chang-Bin [1 ]
Kang, Hae-Na [1 ]
Lee, Soo-Bok [1 ]
机构：
[1] Yonsei Univ, Dept Food & Nutr, Brain Korea Project 21, Seoul 120749, South Korea
来源：
FOOD SCIENCE AND BIOTECHNOLOGY | 2014年 / 23卷 / 04期
基金：
新加坡国家研究基金会;
关键词：
endo-beta-1,3-glucanase; Thermotoga maritima; beta-1,3-glucans; beta-1,3-glucooligosaccharide; anti-fungal; LAMINARINASE; SPP;
D O I：
10.1007/s10068-014-0170-9
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A gene encoding an endo-beta-1,3-glucanase from Thermotoga maritima MSB8 (Tm beta G) was cloned and expressed in Escherichia coli. The purified enzyme produced various beta-1,3-glucooligosaccharides from soluble laminarin, and mainly beta-1,3-glucooligosaccharides smaller than laminaritetrose from insoluble curdlan. The optimum pH and temperature of the enzyme were 5.0 and 80A degrees C, respectively. Tm beta G inhibited the growth of Candida albicans, which indicates that the enzyme could potentially be used as an anti-fungal agent to control invasive infections.
引用
收藏
页码：1243 / 1246
页数：4
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