Oligosaccharide synthesis by endo-β-1,3-glucanase GA from Cellulomonas cellulans

被引:2
|
作者
Buchowiecka, A [1 ]
Bielecki, S [1 ]
机构
[1] Tech Univ Lodz, Inst Tech Biochem, PL-90924 Lodz, Poland
来源
FOOD BIOTECHNOLOGY | 2000年 / 17卷
关键词
D O I
10.1016/S0921-0423(00)80063-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Microbial endo-beta -1,3-glucanase G(A) from Cellulomonas cellulans displaying transglycosylating activity was applied for synthesis of oligosaccharide glycosides. Water-soluble beta -1,3-glucan (laminaran) was used as a donor of oligomeric glycosyl residues and 4-nitrophenyl-beta -D-xylopyranoside served as their acceptor. Transglycosylation reaction carried out at 37 degreesC with 25% acetronitrile as co-solvent resulted in 3 pairs of beta -1,3-, beta -1,4-regioisomers of di-, tri- and tetrasacchride glycosides with total yield 68% after 3 hours process.
引用
收藏
页码:163 / 169
页数:7
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