Oligosaccharide synthesis by endo-β-1,3-glucanase GA from Cellulomonas cellulans

被引：2

作者：

Buchowiecka, A ^{[1
]}

Bielecki, S ^{[1
]}

机构：

[1] Tech Univ Lodz, Inst Tech Biochem, PL-90924 Lodz, Poland

来源：

FOOD BIOTECHNOLOGY | 2000年 / 17卷

关键词：

D O I：

10.1016/S0921-0423(00)80063-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microbial endo-beta -1,3-glucanase G(A) from Cellulomonas cellulans displaying transglycosylating activity was applied for synthesis of oligosaccharide glycosides. Water-soluble beta -1,3-glucan (laminaran) was used as a donor of oligomeric glycosyl residues and 4-nitrophenyl-beta -D-xylopyranoside served as their acceptor. Transglycosylation reaction carried out at 37 degreesC with 25% acetronitrile as co-solvent resulted in 3 pairs of beta -1,3-, beta -1,4-regioisomers of di-, tri- and tetrasacchride glycosides with total yield 68% after 3 hours process.

引用

页码：163 / 169

页数：7

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