Typing of food-borne Listeria monocytogenes by the optimized repetitive extragenic palindrome-based polymerase chain reaction

被引：0

作者：

Pangallo, D ^{[1
]}

Karpísková, R ^{[1
]}

Turna, J ^{[1
]}

Kuchta, T ^{[1
]}

机构：

[1] Inst Food Res, Dept Microbiol & Chem, SK-82475 Bratislava 26, Slovakia

来源：

MICROBIOLOGICA | 2002年 / 25卷 / 04期

关键词：

Listeria monocytogenes; PCR; repetitive extragenic palindrome;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The repetitive extragenic palindrome-based polymerase chain reaction was optimized for typing Listeria monocytogenes by 1) using the QIAamp method to increase the reproducibility of DNA isolation, 2) running PCR with three different DNA concentrations in parallel, 3) using antibody-protected thermostable DNA polymerase to reduce non-specific priming, and 4) using an improved temperature programme to increase the amplification yield. When applied to 42 L. monocytogenes strains isolated from food in the Czech and Slovak republics during 19992000, profiles of 7 - 15 DNA fragments of 330 - 3310 bp were amplified. Based on REP-profiles, strains (serotypes 1/2 and 4) could be divided into 12 groups.

引用

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页码：449 / 454

页数：6

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