Typing of food-borne Listeria monocytogenes by the optimized repetitive extragenic palindrome-based polymerase chain reaction

被引：0
|
作者：
Pangallo, D [1 ]
Karpísková, R [1 ]
Turna, J [1 ]
Kuchta, T [1 ]
机构：
[1] Inst Food Res, Dept Microbiol & Chem, SK-82475 Bratislava 26, Slovakia
来源：
MICROBIOLOGICA | 2002年 / 25卷 / 04期
关键词：
Listeria monocytogenes; PCR; repetitive extragenic palindrome;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The repetitive extragenic palindrome-based polymerase chain reaction was optimized for typing Listeria monocytogenes by 1) using the QIAamp method to increase the reproducibility of DNA isolation, 2) running PCR with three different DNA concentrations in parallel, 3) using antibody-protected thermostable DNA polymerase to reduce non-specific priming, and 4) using an improved temperature programme to increase the amplification yield. When applied to 42 L. monocytogenes strains isolated from food in the Czech and Slovak republics during 19992000, profiles of 7 - 15 DNA fragments of 330 - 3310 bp were amplified. Based on REP-profiles, strains (serotypes 1/2 and 4) could be divided into 12 groups.
引用
收藏
页码：449 / 454
页数：6
相关论文
共 50 条
  • [41] Typing of Ochrobactrum anthropi clinical isolates using automated repetitive extragenic palindromic-polymerase chain reaction DNA fingerprinting and matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry
    Angela Quirino
    Giovanna Pulcrano
    Linda Rametti
    Rossana Puccio
    Nadia Marascio
    Maria Rosaria Catania
    Giovanni Matera
    Maria Carla Liberto
    Alfredo Focà
    BMC Microbiology, 14
  • [42] Typing of Ochrobactrum anthropi clinical isolates using automated repetitive extragenic palindromic-polymerase chain reaction DNA fingerprinting and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry
    Quirino, Angela
    Pulcrano, Giovanna
    Rametti, Linda
    Puccio, Rossana
    Marascio, Nadia
    Catania, Maria Rosaria
    Matera, Giovanni
    Liberto, Maria Carla
    Foca, Alfredo
    BMC MICROBIOLOGY, 2014, 14
  • [43] 16S RIBOSOMAL-RNA-BASED PROBES AND POLYMERASE CHAIN-REACTION METHOD TO DETECT LISTERIA-MONOCYTOGENES CELLS ADDED TO FOODS
    WANG, RF
    CAO, WW
    JOHNSON, MG
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (09) : 2827 - 2831
  • [44] Comparison of automated repetitive-sequence-based polymerase chain reaction and spa typing versus pulsed-field gel electrophoresis for molecular typing of methicillin-resistant Staphylococcus aureus
    Church, Deirdre L.
    Chow, Barbara L.
    Lloyd, Tracie
    Gregson, Daniel B.
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2011, 69 (01) : 30 - 37
  • [45] Evaluation of a polymerase chain reaction-based system for detection of Salmonella enteritidis, Escherichia coli O157:H7, Listeria spp., and Listeria monocytogenes on fresh fruits and vegetables
    Shearer, AEH
    Strapp, CM
    Joerger, RD
    JOURNAL OF FOOD PROTECTION, 2001, 64 (06) : 788 - 795
  • [46] A multiplex polymerase chain reaction assay for rapid detection of Escherichia coli O157:H7, Listeria monocytogenes, Salmonella Typhimurium and Campylobacter jejuni in artificially contaminated food samples
    Wang, H
    Slavik, MF
    JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY, 2005, 13 (03): : 213 - 223
  • [47] SELECTION OF OPTIMAL PRIMER SETS FOR USE IN A DUPLEX SYBR GREEN-BASED, REAL-TIME POLYMERASE CHAIN REACTION PROTOCOL FOR THE DETECTION OF LISTERIA MONOCYTOGENES AND STAPHYLOCCOCUS AUREUS IN FOODS
    Martinon, A.
    Wilkinson, M. G.
    JOURNAL OF FOOD SAFETY, 2011, 31 (03) : 297 - 312
  • [48] Polydopamine-modified 3D flower-like ZnMoO4 integrated MXene-based label-free electrochemical immunosensor for the food-borne pathogen Listeria monocytogenes detection in milk and seafood
    Baskaran, Nareshkumar
    Sakthivel, Rajalakshmi
    Karthik, Chimatahalli Shanthakumar
    Lin, Yu-Chien
    Liu, Xinke
    Wen, Hsiao-Wei
    Yang, Wei
    Chung, Ren-Jei
    Talanta, 2025, 282
  • [49] Detection of Salmonella spp., Escherichia coli O157, Listeria monocytogenes and Campylobacter spp. in chicken samples by multiplex polymerase chain reaction and hybridization using the genegen major food pathogens detection kit
    Vollenhofer-Schrumpf, S
    Buresch, R
    Unger, G
    Stahl, N
    Fränzl, G
    Schinkinger, M
    JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY, 2005, 13 (03): : 148 - 176
  • [50] Detection of Pathogenic Serogroups and Virulence Genes in Listeria monocytogenes Strains Isolated from Beef and Beef Products Retailed in Gauteng Province, South Africa, Using Phenotypic and Polymerase Chain Reaction (PCR)-Based Methods
    Gana, James
    Gcebe, Nomakorinte
    Moerane, Rebone
    Ngoshe, Yusuf B.
    Moabelo, Khomotso
    Adesiyun, Abiodun A.
    INTERNATIONAL JOURNAL OF MICROBIOLOGY, 2024, 2024
12345