Typing of Haemophilus paragallinarum strains by using enterobacterial repetitive intergenic consensus-based polymerase chain reaction

被引：17
|
作者：
Soriano, VE
Téllez, G
Hargis, BM
Newberry, L
Salgado-Miranda, C
Vázquez, JC
机构：
[1] UAEM, CIESA, FMVZ, Toluca 50000, Mexico
[2] Univ Arkansas, Ctr Excellence Poultry Sci, Div Agr, John Kirkpatrick Skeeles Poultry Hlth Res Lab, Fayetteville, AR 72701 USA
来源：
AVIAN DISEASES | 2004年 / 48卷 / 04期
关键词：
Haemophilus paragallinarum; ERIC-PCR; fingerprinting; typing;
D O I：
10.1637/7137
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) technique was used for fingerprinting of reference strains and Mexican isolates of Haemophilus paragallinarum. A total of nine ERIC patterns were given by the nine serovar reference strains of this bacteria. Two Modesto (C-2) reference strains from different sources showed the same ERIC pattern. Seventeen ERIC patterns were obtained among 29 Mexican isolates included in the study, belonging to serovars prevalent in Mexico (A-1, A-2, B-1, and C-2). Obtained results indicate that the ERIC-PCR technique could be used as a molecular laboratory tool for subryping of H. paragallinarum.
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页码：890 / 895
页数：6
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