Studies on inhibition mechanism of transcription factor NF-κB and DNA binding by chelator pyrogallol red on the basis of its interaction with metal ions

被引：2
|
作者：
Sharma, R. K. [1 ]
Chelladurai, C. [1 ]
Tiwari, A. D. [1 ]
Rajor, H. K. [1 ]
Mehta, S. [1 ]
Otsuka, M. [2 ]
机构：
[1] Univ Delhi, Dept Chem, Green Chem Network Ctr, Delhi 7, India
[2] Kumamoto Univ, Fac Med & Pharmaceut Sci, Kumamoto 8620973, Japan
来源：
BIOORGANIC & MEDICINAL CHEMISTRY | 2008年 / 16卷 / 19期
关键词：
pyrogallol red; zinc; NF-kappa B-DNA binding; H-1; NMR; IR; TGA and EMSA;
D O I：
10.1016/j.bmc.2008.08.038
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recent studies have shown that the zinc influences the DNA binding of transcription factor NF-kappa B. So the zinc-interaction of pyrogallol red (PR) an inhibitor of NF-kappa B-DNA binding was studied by isolating the zinc-PR complex. The complex was characterized using IR, UV-visible, H-1 NMR, and thermal studies. Binding sites of PR were investigated by molecular modeling using MM+ and PM3 methods and by generating molecular electrostatic map. These studies have confirmed the role of metal ion chelation in the inhibition of NF-kappa B-DNA binding by PR. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：9018 / 9022
页数：5
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