Studies on inhibition mechanism of transcription factor NF-κB and DNA binding by chelator pyrogallol red on the basis of its interaction with metal ions

被引：2

作者：

Sharma, R. K. ^{[1
]}

Chelladurai, C. ^{[1
]}

Tiwari, A. D. ^{[1
]}

Rajor, H. K. ^{[1
]}

Mehta, S. ^{[1
]}

Otsuka, M. ^{[2
]}

机构：

[1] Univ Delhi, Dept Chem, Green Chem Network Ctr, Delhi 7, India

[2] Kumamoto Univ, Fac Med & Pharmaceut Sci, Kumamoto 8620973, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2008年 / 16卷 / 19期

关键词：

pyrogallol red; zinc; NF-kappa B-DNA binding; H-1; NMR; IR; TGA and EMSA;

D O I：

10.1016/j.bmc.2008.08.038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies have shown that the zinc influences the DNA binding of transcription factor NF-kappa B. So the zinc-interaction of pyrogallol red (PR) an inhibitor of NF-kappa B-DNA binding was studied by isolating the zinc-PR complex. The complex was characterized using IR, UV-visible, H-1 NMR, and thermal studies. Binding sites of PR were investigated by molecular modeling using MM+ and PM3 methods and by generating molecular electrostatic map. These studies have confirmed the role of metal ion chelation in the inhibition of NF-kappa B-DNA binding by PR. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：9018 / 9022

页数：5

共 44 条

[21] DNA-binding ability of NF-κB is affected differently by ERα and ERβ and its activation results in inhibition of estrogen responsiveness
Guzeloglu-Kayisli, Ozlem
Halls, Gulden
Taskiran, Sarper
Kayish, Umit A.
Arici, Aydin
REPRODUCTIVE SCIENCES, 2008, 15 (05) : 493 - 505
[22] DNA-Binding Ability of NF-κB is Affected Differently by ERα and ERβ and Its Activation Results in Inhibition of Estrogen Responsiveness
Ozlem Guzeloglu-Kayisli
Gulden Halis
Sarper Taskiran
Umit A. Kayisli
Aydin Arici
Reproductive Sciences, 2008, 15 : 493 - 505
[23] INTERACTION OF TRANSCRIPTION FACTORS OF NF-KAPPA-B/REL FAMILY WITH DNA RESULTS IN ITS SIGNIFICANT BENDING AT THE BINDING-SITE
KUPRASH, DV
NEDOSPASOV, SA
DOKLADY AKADEMII NAUK SSSR, 1992, 322 (02): : 421 - 426
[24] Histone deacetylase inhibition down-regulates cyclin D1 transcription by inhibiting NF-κB/p65 DNA binding
Hu, Jing
Colburn, Nancy H.
CANCER RESEARCH, 2006, 66 (08)
[25] Induction of cyclooxygenase-2 by platelet-derived growth factor (PDGF) and its inhibition by dexamethasone are independent of NF-κB/IκB transcription factors
Margarete Goppelt-Struebe
Margot Rehm
Heinz-Juergen Schaefers
Naunyn-Schmiedeberg's Archives of Pharmacology, 2000, 361 : 636 - 645
[26] Induction of cyclooxygenase-2 by platelet-derived growth factor (PDGF) and its inhibition by dexamethasone are independent of NF-κB/IκB transcription factors
Goppelt-Struebe, M
Rehm, M
Schaefers, HJ
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2000, 361 (06) : 636 - 645
[27] Thrombin activates transcription factors Sp1, NF-κB and CREB.: Importance of the nuclear protein extraction procedure in the assessment of transcription factor DNA binding activities
Edmead, C
Kanthou, C
Benzakour, O
THROMBOSIS AND HAEMOSTASIS, 1999, : 299 - 299
[28] Structural studies on 24P-IκBα peptide derived from a human IκB-α protein related to the inhibition of the activity of the transcription factor NF-κB
Pons, Julien
Evrard-Todeschi, Nathalie
Bertho, Gildas
Gharbi-Benarous, Josyane
Sonois, Vanessa
Benarous, Richard
Girault, Jean-Pierre
BIOCHEMISTRY, 2007, 46 (11) : 2958 - 2972
[29] Biological evaluation, chelation, and molecular modeling studies of novel metal-chelating inhibitors of NF-κB-DNA binding:: Structure activity relationships
Sharma, RK
Chopra, S
Sharma, SD
Pande, V
Ramos, MJ
Meguro, K
Inoue, JI
Otsuka, M
JOURNAL OF MEDICINAL CHEMISTRY, 2006, 49 (12) : 3595 - 3601
[30] Listeria monocytogenes infection of Caco-2 human epithelial cells induces activation of transcription factor NF-κB/Rel-like DNA binding activities
Hauf, N
Goebel, W
Fiedler, F
Kuhn, M
FEMS MICROBIOLOGY LETTERS, 1999, 178 (01) : 117 - 122

← 1 2 3 4 5 →