Studies on inhibition mechanism of transcription factor NF-κB and DNA binding by chelator pyrogallol red on the basis of its interaction with metal ions

被引：2
|
作者：
Sharma, R. K. [1 ]
Chelladurai, C. [1 ]
Tiwari, A. D. [1 ]
Rajor, H. K. [1 ]
Mehta, S. [1 ]
Otsuka, M. [2 ]
机构：
[1] Univ Delhi, Dept Chem, Green Chem Network Ctr, Delhi 7, India
[2] Kumamoto Univ, Fac Med & Pharmaceut Sci, Kumamoto 8620973, Japan
来源：
BIOORGANIC & MEDICINAL CHEMISTRY | 2008年 / 16卷 / 19期
关键词：
pyrogallol red; zinc; NF-kappa B-DNA binding; H-1; NMR; IR; TGA and EMSA;
D O I：
10.1016/j.bmc.2008.08.038
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recent studies have shown that the zinc influences the DNA binding of transcription factor NF-kappa B. So the zinc-interaction of pyrogallol red (PR) an inhibitor of NF-kappa B-DNA binding was studied by isolating the zinc-PR complex. The complex was characterized using IR, UV-visible, H-1 NMR, and thermal studies. Binding sites of PR were investigated by molecular modeling using MM+ and PM3 methods and by generating molecular electrostatic map. These studies have confirmed the role of metal ion chelation in the inhibition of NF-kappa B-DNA binding by PR. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：9018 / 9022
页数：5
相关论文
共 44 条
  • [1] Regions of IκBα that are critical for its inhibition of NF-κB•DNA interaction fold upon binding to NF-κB
    Truhlar, Stephanie M. E.
    Torpey, Justin W.
    Komives, Elizabeth A.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (50) : 18951 - 18956
  • [2] Inhibition of transcription factor NF-κB by sesquiterpene lactones:: a proposed molecular mechanism of action
    Rüngeler, P
    Castro, V
    Mora, G
    Gören, N
    Vichnewski, W
    Pahl, HL
    Merfort, I
    Schmidt, TJ
    BIOORGANIC & MEDICINAL CHEMISTRY, 1999, 7 (11) : 2343 - 2352
  • [3] NBPF is a potential DNA-binding transcription factor that is directly regulated by NF-κB
    Zhou, Fei
    Xing, Yujun
    Xu, Xinhui
    Yang, Yang
    Zhang, Jinwei
    Ma, Zhengliang
    Wang, Jinke
    INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2013, 45 (11): : 2479 - 2490
  • [4] Interaction of the IκBα C-terminal PEST Sequence with NF-κB: Insights into the Inhibition of NF-κB DNA Binding by IκBα
    Sue, Shih-Che
    Dyson, H. Jane
    JOURNAL OF MOLECULAR BIOLOGY, 2009, 388 (04) : 824 - 838
  • [5] Molecular interactions between nuclear factor κB (NF-κB) transcription factors and a PNA-DNA chimera mimicking NF-κB binding sites
    Romanelli, A
    Pedone, C
    Saviano, M
    Bianchi, N
    Borgatti, M
    Mischiati, C
    Gambari, R
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (23): : 6066 - 6075
  • [6] Virtual Screening against p50 NF-κB Transcription Factor for the Identification of Inhibitors of the NF-κB-DNA Interaction and Expression of NF-κB Upregulated Genes
    Piccagli, Laura
    Fabbri, Enrica
    Borgatti, Monica
    Bianchi, Nicoletta
    Bezzerri, Valentino
    Mancini, Irene
    Nicolis, Elena
    Dechecchi, Cristina M.
    Lampronti, Ilaria
    Cabrini, Giulio
    Gambari, Roberto
    CHEMMEDCHEM, 2009, 4 (12) : 2024 - 2033
  • [7] Spectroscopic studies of the interaction of Synechoccocus SmtB, a zinc metalloregulatory transcription factor, with DNA and metal ions
    VanZile, ML
    Giedroc, DP
    BIOPHYSICAL JOURNAL, 1999, 76 (01) : A133 - A133
  • [8] Interaction of the human NF-κB p52 transcription factor with DNA-PNA hybrids mimicking the NF-κB binding sites of the human immunodeficiency virus type 1 promoter
    Mischiati, C
    Borgatti, M
    Bianchi, N
    Rutigliano, C
    Tomassetti, M
    Feriotto, G
    Gambari, R
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (46) : 33114 - 33122
  • [9] The Expression and DNA-Binding Activity of NF-κB Nuclear Transcription Factor in the Tumors of Patients with Breast Cancer
    E. S. Gershtein
    A. M. Scherbakov
    A. M. Platova
    G. Yu. Tchemeris
    V. P. Letyagin
    N. E. Kushlinskii
    Bulletin of Experimental Biology and Medicine, 2010, 150 : 71 - 74
  • [10] The Expression and DNA-Binding Activity of NF-κB Nuclear Transcription Factor in the Tumors of Patients with Breast Cancer
    Gershtein, E. S.
    Scherbakov, A. M.
    Platova, A. M.
    Tchemeris, G. Yu.
    Letyagin, V. P.
    Kushlinskii, N. E.
    BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2010, 150 (01) : 71 - 74
12345