A rapid, simple, specific liquid chromatographic-electrospray mass spectrometry method for the determination of finasteride in human plasma and its application to pharmacokinetic study

被引:16
|
作者
Guo, Fanc-Qiu
Huang, Lan-Fang [1 ]
Wong, Kin Ping
Dai, Yun-Hui
Li, Ya-Wen
Liang, Yi-Zeng
Huang, Ke-Long
Zhong, Ke-Jun
Wu, Ming-Han
机构
[1] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Peoples R China
[2] Calif State Univ Fresno, Dept Biol, Coll Sci & Math, Fresno, CA 93740 USA
[3] Changde Cigarette Factory, Ctr Tech, Changde 415000, Peoples R China
关键词
finasteride; liquid chromatography-mass spectrometry (LC-MS); electrospray ionization (ESI); pharmacokinetic;
D O I
10.1016/j.jpba.2006.10.024
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A fast, accurate, sensitive, selective and reliable method using reversed-phase high-performance liquid chromatography-mass spectrometry coupling with an electrospray ionization interface was developed and validated for the determination of finasteride in human plasma. After deprotienation with acetonitrile, centrifugation, evaporation to dryness and dissolving in mobile phase, satisfactory separation was achieved on a Hypersil-Keystone C(18) reversed-phase column using a mobile phase consisting of acetonitrile-water (46:54, v/v), 0.1% acetic acid and 0.1% trifluoracetic acid. Carbamazepine (IS) was used as internal standard. This method involved the use of the [M+H](+) ions of finasteride and IS at m/z 373 and 237 with the selective ion monitoring (SIM) mode. The calibration curve was linear in the range of 0.2-120 ng ml(-1). The limit of quantification for finasteride in plasma was 0.2 ng ml(-1) with good accuracy and precision. The intra-assay precision and accuracy were in the range of 2.1-11.2% and -1.3% to 8.5%, respectively. The inter-assay precision and accuracy were in the order of 3.4-12.1% and -1.5% to 11.5%, respectively. The mean sample extract recoveries of the method were higher than 85% and 74% for finasteride and internal standard (IS), respectively. The assay has been successfully used to estimate the pharmacokinetics of finasteride after oral administration of a 5 mg tablet of finasteride to 24 healthy volunteers. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:1507 / 1513
页数:7
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