Isolation, characterization and complete genome sequence of Phaxl: a phage of Escherichia coli O157: H7

被引：32

作者：

Shahrbabak, Salehe Sabouri ^{[1
,2
,3
]}

Khodabandehlou, Zahra ^{[1
,2
]}

Shahverdi, Ahmad Reza ^{[1
,2
]}

Skurnik, Mikael ^{[4
,5
]}

Ackermann, Hans-Wolfgang ^{[6
]}

Varjosalo, Markku ^{[7
]}

Yazdi, Mojtaba Tabatabaei ^{[1
,2
]}

Sepehrizadeh, Zargham ^{[1
,2
]}

机构：

[1] Univ Tehran Med Sci, Fac Pharm, Dept Pharmaceut Biotechnol, Tehran, Iran

[2] Univ Tehran Med Sci, Fac Pharm, Biotechnol Res Ctr, Tehran, Iran

[3] Kerman Univ Med Sci, Pharmaceut Res Ctr, Kerman, Iran

[4] Univ Helsinki, Dept Bacteriol & Immunol, Haartman Inst, Helsinki, Finland

[5] Univ Helsinki, Cent Hosp Lab, Helsinki, Finland

[6] Univ Laval, Fac Med, Dept Microbiol Immunol & Infectiol, Quebec City, PQ G1X 4C6, Canada

[7] Univ Helsinki, Inst Biotechnol, Helsinki, Finland

来源：

MICROBIOLOGY-SGM | 2013年 / 159卷

关键词：

SALMONELLA-ENTERICA; SEROTYPE O157-H7; BACTERIOPHAGE; IDENTIFICATION; TRANSCRIPTION; BIOCONTROL; PREDICTION; RESISTANCE; RECEPTOR; CATTLE;

D O I：

10.1099/mic.0.063776-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacteriophages are considered as promising biological agents for the control of infectious diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, Phaxl, was isolated from a sewage sample in Iran. Morphological studies by transmission electron microscopy showed that it has an icosahedral capsid of 85-86 nm and a contractile tail of 115x15 nm. Phaxl contains dsDNA composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins. In MS analysis of phage particles, 92 structural proteins were identified. Phaxl lyses Escherichia coli O157 : H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent period of 40 min, and has a burst size of about 420 particles per cell. Phaxl is a member of the genus 'Viunalikevirus' of the family Myoviridae and is specific for E. coli O157 : H7.

引用

页码：1629 / 1638

页数：10

共 50 条

[31] The criterion for selecting effective phage for Escherichia coli O157:H7 control
Yoichi, M
Morita, M
Mizoguchi, K
Fischer, CR
Unno, H
Tanji, Y
BIOCHEMICAL ENGINEERING JOURNAL, 2004, 19 (03) : 221 - 227
[32] Toward rational control of Escherichia coli O157:H7 by a phage cocktail
Y. Tanji
T. Shimada
M. Yoichi
K. Miyanaga
K. Hori
H. Unno
Applied Microbiology and Biotechnology, 2004, 64 : 270 - 274
[33] Genome evolution in major Escherichia coli O157:H7 lineages
Yongxiang Zhang
Chad Laing
Marina Steele
Kim Ziebell
Roger Johnson
Andrew K Benson
Eduardo Taboada
Victor PJ Gannon
BMC Genomics, 8
[34] The complete DNA sequence and analysis of the large virulence plasmid of Escherichia coli O157:H7
Burland, V
Shao, Y
Perna, NT
Plunkett, G
Sofia, HJ
Blattner, FR
NUCLEIC ACIDS RESEARCH, 1998, 26 (18) : 4196 - 4204
[35] Toward rational control of Escherichia coli O157:H7 by a phage cocktail
Tanji, Y
Shimada, T
Yoichi, M
Miyanaga, K
Hori, K
Unno, H
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2004, 64 (02) : 270 - 274
[36] Escherichia coli Serotype O157:H7 PA20R2R Complete Genome Sequence
Uhlich, Gaylen A.
Paoli, George C.
Kanrar, Siddhartha
MICROBIOLOGY RESOURCE ANNOUNCEMENTS, 2020, 9 (50):
[37] Complete Genome Sequence of an Escherichia coli O157:H7 Strain Isolated from a Super-Shedder Steer
Teng, Lin
Ginn, Amber
Jeon, Soojin
Kang, Minyoung
Jeong, Kwangcheol Casey
GENOME ANNOUNCEMENTS, 2016, 4 (02)
[38] Improvement of selectivity for isolation of Escherichia coli O157:H7 from generic Escherichia coli
Rhee, MS
Gray, PM
Kang, DH
JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY, 2002, 10 (01): : 27 - 35
[39] Immunological characterization of Escherichia coli O157:H7 intimin γ1
Son, WG
Graham, TA
Gannon, VPJ
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2002, 9 (01) : 46 - 53
[40] Detection and characterization of the fimA gene of Escherichia coli O157:H7
Li, BG
Koch, WH
Cebula, TA
MOLECULAR AND CELLULAR PROBES, 1997, 11 (06) : 397 - 406

← 1 2 3 4 5 →