Isolation, characterization and complete genome sequence of Phaxl: a phage of Escherichia coli O157: H7

被引：32

作者：

Shahrbabak, Salehe Sabouri ^{[1
,2
,3
]}

Khodabandehlou, Zahra ^{[1
,2
]}

Shahverdi, Ahmad Reza ^{[1
,2
]}

Skurnik, Mikael ^{[4
,5
]}

Ackermann, Hans-Wolfgang ^{[6
]}

Varjosalo, Markku ^{[7
]}

Yazdi, Mojtaba Tabatabaei ^{[1
,2
]}

Sepehrizadeh, Zargham ^{[1
,2
]}

机构：

[1] Univ Tehran Med Sci, Fac Pharm, Dept Pharmaceut Biotechnol, Tehran, Iran

[2] Univ Tehran Med Sci, Fac Pharm, Biotechnol Res Ctr, Tehran, Iran

[3] Kerman Univ Med Sci, Pharmaceut Res Ctr, Kerman, Iran

[4] Univ Helsinki, Dept Bacteriol & Immunol, Haartman Inst, Helsinki, Finland

[5] Univ Helsinki, Cent Hosp Lab, Helsinki, Finland

[6] Univ Laval, Fac Med, Dept Microbiol Immunol & Infectiol, Quebec City, PQ G1X 4C6, Canada

[7] Univ Helsinki, Inst Biotechnol, Helsinki, Finland

来源：

MICROBIOLOGY-SGM | 2013年 / 159卷

关键词：

SALMONELLA-ENTERICA; SEROTYPE O157-H7; BACTERIOPHAGE; IDENTIFICATION; TRANSCRIPTION; BIOCONTROL; PREDICTION; RESISTANCE; RECEPTOR; CATTLE;

D O I：

10.1099/mic.0.063776-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacteriophages are considered as promising biological agents for the control of infectious diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, Phaxl, was isolated from a sewage sample in Iran. Morphological studies by transmission electron microscopy showed that it has an icosahedral capsid of 85-86 nm and a contractile tail of 115x15 nm. Phaxl contains dsDNA composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins. In MS analysis of phage particles, 92 structural proteins were identified. Phaxl lyses Escherichia coli O157 : H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent period of 40 min, and has a burst size of about 420 particles per cell. Phaxl is a member of the genus 'Viunalikevirus' of the family Myoviridae and is specific for E. coli O157 : H7.

引用

页码：1629 / 1638

页数：10

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