Chloramphenicol acetyltransferase-a new selectable marker in stable nuclear transformation of the red alga Cyanidioschyzon merolae

被引:12
|
作者
Zienkiewicz, Maksymilian [1 ]
Krupnik, Tomasz [1 ]
Drozak, Anna [1 ]
Golke, Anna [1 ]
Romanowska, Elzbieta [1 ]
机构
[1] Warsaw Univ, Warsaw, Poland
关键词
Cyanidioschyzon merolae; Chloramphenicol acetyltransferase (CAT) stable genome transformation; MITOCHONDRIAL PROTEIN-SYNTHESIS; TRANSIENT GENE-EXPRESSION; TRANSCRIPTION FACTOR; ESCHERICHIA-COLI; PSBQ PROTEIN; CHLOROPLAST; SEQUENCE; GENOME; CLEAVAGE; REVEALS;
D O I
10.1007/s00709-015-0936-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae-a new model organism, which offers unique opportunities for studding the mitochondrial and plastid physiology as well as various evolutionary, structural, and functional features of the photosynthetic apparatus.
引用
收藏
页码:587 / 596
页数:10
相关论文
共 35 条
  • [1] Chloramphenicol acetyltransferase—a new selectable marker in stable nuclear transformation of the red alga Cyanidioschyzon merolae
    Maksymilian Zienkiewicz
    Tomasz Krupnik
    Anna Drożak
    Anna Golke
    Elżbieta Romanowska
    [J]. Protoplasma, 2017, 254 : 587 - 596
  • [2] A cotransformation system of the unicellular red alga Cyanidioschyzon merolae with blasticidin S deaminase and chloramphenicol acetyltransferase selectable markers
    Fujiwara, Takayuki
    Hirooka, Shunsuke
    Miyagishima, Shin-ya
    [J]. BMC PLANT BIOLOGY, 2021, 21 (01)
  • [3] A cotransformation system of the unicellular red alga Cyanidioschyzon merolae with blasticidin S deaminase and chloramphenicol acetyltransferase selectable markers
    Takayuki Fujiwara
    Shunsuke Hirooka
    Shin-ya Miyagishima
    [J]. BMC Plant Biology, 21
  • [4] Development of nuclear transformation in red alga Cyanidioschyzon merolae 10D
    Ohnuma, M
    Yokoyama, T
    Inouye, T
    Sekine, Y
    Tanaka, K
    [J]. PLANT AND CELL PHYSIOLOGY, 2006, 47 : S189 - S189
  • [5] Development of nuclear transformation in red alga Cyanidioschyzon merolae 10D
    Ohnuma, Mio
    Yokoyama, Takashi
    Inouye, Takayuki
    Sekine, Yasuhiko
    Tanaka, Kan
    [J]. PLANT AND CELL PHYSIOLOGY, 2007, 48 : S182 - S182
  • [6] Construction of a Selectable Marker Recycling System and the Use in Epitope Tagging of Multiple Nuclear Genes in the Unicellular Red Alga Cyanidioschyzon merolae
    Takemura, Tokiaki
    Imamura, Sousuke
    Kobayashi, Yuki
    Tanaka, Kan
    [J]. PLANT AND CELL PHYSIOLOGY, 2018, 59 (11) : 2308 - 2316
  • [7] Chloramphenicol acetyltransferase as selectable marker for plastid transformation
    Weimin Li
    Stephanie Ruf
    Ralph Bock
    [J]. Plant Molecular Biology, 2011, 76 : 443 - 451
  • [8] Chloramphenicol acetyltransferase as selectable marker for plastid transformation
    Li, Weimin
    Ruf, Stephanie
    Bock, Ralph
    [J]. PLANT MOLECULAR BIOLOGY, 2011, 76 (3-5) : 443 - 451
  • [9] Stable expression of a GFP-reporter gene in the red alga Cyanidioschyzon merolae
    Watanabe, Satoru
    Sato, Jun
    Imamura, Sousuke
    Ohnuma, Mio
    Ohoba, Yusaku
    Chibazakura, Taku
    Tanaka, Kan
    Yoshikawa, Hirofumi
    [J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2014, 78 (01) : 175 - 177
  • [10] Nucleotide sequences of the plastid and nuclear chromosome I of the unicellular red alga Cyanidioschyzon merolae
    Kawashima, T
    Amano, N
    Higuchi, S
    Minezaki, Y
    Clowney, L
    Ishijima, SA
    Koike, H
    Aramaki, H
    Nunoshiba, T
    Kawamoto, T
    Yokoyama, K
    Kimura, S
    Makino, K
    Suzuki, M
    [J]. PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 2002, 78 (10): : 299 - 304