Chloramphenicol acetyltransferase-a new selectable marker in stable nuclear transformation of the red alga Cyanidioschyzon merolae

被引:12
|
作者
Zienkiewicz, Maksymilian [1 ]
Krupnik, Tomasz [1 ]
Drozak, Anna [1 ]
Golke, Anna [1 ]
Romanowska, Elzbieta [1 ]
机构
[1] Warsaw Univ, Warsaw, Poland
关键词
Cyanidioschyzon merolae; Chloramphenicol acetyltransferase (CAT) stable genome transformation; MITOCHONDRIAL PROTEIN-SYNTHESIS; TRANSIENT GENE-EXPRESSION; TRANSCRIPTION FACTOR; ESCHERICHIA-COLI; PSBQ PROTEIN; CHLOROPLAST; SEQUENCE; GENOME; CLEAVAGE; REVEALS;
D O I
10.1007/s00709-015-0936-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae-a new model organism, which offers unique opportunities for studding the mitochondrial and plastid physiology as well as various evolutionary, structural, and functional features of the photosynthetic apparatus.
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页码:587 / 596
页数:10
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