Chloramphenicol acetyltransferase-a new selectable marker in stable nuclear transformation of the red alga Cyanidioschyzon merolae

被引：12

作者：

Zienkiewicz, Maksymilian ^{[1
]}

Krupnik, Tomasz ^{[1
]}

Drozak, Anna ^{[1
]}

Golke, Anna ^{[1
]}

Romanowska, Elzbieta ^{[1
]}

机构：

[1] Warsaw Univ, Warsaw, Poland

来源：

PROTOPLASMA | 2017年 / 254卷 / 01期

关键词：

Cyanidioschyzon merolae; Chloramphenicol acetyltransferase (CAT) stable genome transformation; MITOCHONDRIAL PROTEIN-SYNTHESIS; TRANSIENT GENE-EXPRESSION; TRANSCRIPTION FACTOR; ESCHERICHIA-COLI; PSBQ PROTEIN; CHLOROPLAST; SEQUENCE; GENOME; CLEAVAGE; REVEALS;

D O I：

10.1007/s00709-015-0936-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In this study, we have shown the applicability of chloramphenicol acetyltransferase as a new and convenient selectable marker for stable nuclear transformation as well as potential chloroplast transformation of Cyanidioschyzon merolae-a new model organism, which offers unique opportunities for studding the mitochondrial and plastid physiology as well as various evolutionary, structural, and functional features of the photosynthetic apparatus.

引用

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页码：587 / 596

页数：10

共 35 条

[21] Identification of highly-disrupted tRNA genes in nuclear genome of the red alga, Cyanidioschyzon merolae 10D
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SCIENTIFIC REPORTS, 2013, 3
[22] Identification of highly-disrupted tRNA genes in nuclear genome of the red alga, Cyanidioschyzon merolae 10D
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[23] Characterization of the Nuclear- and Plastid-Encoded secA-Homologous Genes in the Unicellular Red Alga Cyanidioschyzon merolae
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[24] Construction of a URA5.3 deletion strain of the unicellular red alga Cyanidioschyzon merolae: A backgroundless host strain for transformation experiments
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[25] STABLE NUCLEAR TRANSFORMATION OF CHLAMYDOMONAS-REINHARDTII BY USING A CHLAMYDOMONAS-REINHARDTII GENE AS THE SELECTABLE MARKER
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PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (06) : 2087 - 2091
[26] A Codon-Optimized Bacterial Antibiotic Gene Used as Selection Marker for Stable Nuclear Transformation in the Marine Red Alga Pyropia yezoensis
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[27] A Codon-Optimized Bacterial Antibiotic Gene Used as Selection Marker for Stable Nuclear Transformation in the Marine Red Alga Pyropia yezoensis
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[28] Nuclear-encoded chloroplast RNA polymerase sigma factor SIG2 activates chloroplast-encoded phycobilisome genes in a red alga, Cyanidioschyzon merolae
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[29] Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker
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[30] Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker
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PLANT MOLECULAR BIOLOGY, 2016, 90 (4-5) : 403 - 418

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