Stable expression of a GFP-reporter gene in the red alga Cyanidioschyzon merolae

被引：7
|
作者：
Watanabe, Satoru [1 ]
Sato, Jun [1 ]
Imamura, Sousuke [2 ,3 ]
Ohnuma, Mio [4 ]
Ohoba, Yusaku [1 ]
Chibazakura, Taku [1 ]
Tanaka, Kan [2 ,3 ]
Yoshikawa, Hirofumi [1 ,3 ]
机构：
[1] Tokyo Univ Agr, Dept Biosci, Tokyo, Japan
[2] Tokyo Inst Technol, Chem Resources Lab, Yokohama, Kanagawa 227, Japan
[3] Japan Sci & Technol Agcy JST, CREST, Saitama, Japan
[4] Rikkyo Univ, Coll Sci, Tokyo 171, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2014年 / 78卷 / 01期
关键词：
Cyanidioschyzon merolae; transformation; APCC; green fluorescent protein (GFP); SEQUENCE; 10D; GENOME; SYSTEM;
D O I：
10.1080/09168451.2014.877823
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The unicellular red alga Cyanidioschyzon merolae is used as a model organism to investigate the basic architecture of photosynthetic eukaryotes. We established a stable expression system for the green fluorescent protein fused with the phycocyanin-associated rod linker (APCC) protein in C. merolae, which was clearly localized on the plastid. This system should be useful in the genetic engineering of C. merolae.
引用
收藏
页码：175 / 177
页数：3
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