Synthesis of oligonucleotides containing 5′-homo-4′-selenouridine derivative and its increased resistance against nuclease

被引：1
|
作者：
Yu, Jinha [1 ,2 ,3 ]
Kim, Ji Won [1 ]
Chandra, Girish [1 ,4 ]
Saito-Tarashima, Noriko [5 ]
Nogi, Yuhei [5 ]
Ota, Masashi [5 ]
Minakawa, Noriaki [5 ]
Jeong, Lak Shin [1 ]
机构：
[1] Seoul Natl Univ, Coll Pharm, Seoul 08826, South Korea
[2] Ewha Womans Univ, Coll Pharm, Seoul 03760, South Korea
[3] Ewha Womans Univ, Grad Sch Pharmaceut Sci, Seoul 03760, South Korea
[4] Cent Univ South Bihar, Gaya 824236, Bihar, India
[5] Tokushima Univ, Grad Sch Pharmaceut Sci, Shomachi 1-78-1, Tokushima 7708505, Japan
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2023年 / 83卷
基金：
新加坡国家研究基金会;
关键词：
5 '-Homo-4 '-Selenonucleoside; Oligonucleotide; Nuclease resistance; Thermostability; 1ST SYNTHESIS; ANTISENSE; HYBRIDIZATION;
D O I：
10.1016/j.bmcl.2023.129172
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
As technologies using RNA or DNA have been developed, various chemical modifications of nucleosides have been attempted to increase the stability of oligonucleotides. Since it is known that 2 '-OMe-modification greatly contributes to increasing the stability of oligonucleotides, we added 2 '-OMe to our previously developed 4 '-selenonucleoside and 5 '-homo-4 '-selenonucleoside as the modified monomers for oligonucleotide: 2 '-methoxy-4 '-selenouridine (2 '-OMe-4 '-Se-U) and 5 '-homo-2 '-methoxy-4 '-selenouridine (5 '-homo-2 '-OMe-4 '-Se-U). We synthesized oligonucleotides containing the chemically modified 4 '-selenouridine and evaluated their thermal stability and nuclease resistance. In conclusion, the nuclease stability of the oligonucleotide containing 5 '-homo-2 '-OMe-4 '-selenouridine increased while its thermal stability decreased.
引用
收藏
页数：5
相关论文
共 50 条
12345