Corneal layer thickness in keratoconus using optical coherence tomography

BackgroundTo evaluate the thicknesses of different corneal layers for identifying keratoconus (KCN) and subclinical keratoconus (SKCN) using spectral-domain optical coherence tomography (SD-OCT).MethodsThis prospective study analyzed 60 eyes with KCN, 48 eyes with SKCN, and 53 normal eyes. The central corneal thickness (CCT) and thicknesses of the epithelium, Bowman, stroma, and Descemet-endothelium layers were measured using SD-OCT. One way analysis of variance and the area under the curve (AUC) were used to evaluate the parameters. The Delong method was used to compare AUCs.ResultsIn KCN, CCT and thicknesses of epithelium, Bowman, stroma, and Descemet-endothelium layers were 495.5 +/- 41.7, 52.6 +/- 6.4,11.5 +/- 1.4, 415.5 +/- 38.9, and 12.3 +/- 1.7 mu m, respectively. These thickness values were respectively 524.5 +/- 33.3, 56.8 +/- 6.8, 11.5 +/- 1.6, 439.8 +/- 30.6, and 12.4 +/- 1.7 mu m in SKCN and 563.8 +/- 37.9, 57.7 +/- 6.9, 12.2 +/- 1.6, 469.5 +/- 33.7, and 12.8 +/- 2.1 mu m in normal group. Total cornea and stroma in KCN and SKCN, and epithelium in KCN were significantly thinner compared to the normal group (P < 0.001). The highest AUC values were observed for CCT in KCN (AUC 0.90) and SKCN (AUC 0.782). The diagnostic accuracy was significantly higher for stromal thickness in KCN (sensitivity 81.7%, specificity 73.6%, AUC 0.871) and SKCN (sensitivity 80.0%, specificity 56.6%, AUC 0.751) than other individual corneal layers (Delong, P < 0.001) .ConclusionCCT can accurately distinguish keratoconus from normal eyes. However, central corneal stromal thinning was the most sensitive diagnostic index for early detection of SKCN. Developing standardized stromal maps may be helpful for detecting SKCN.