Analyzing engram reactivation and long-range connectivity

被引：0
|
作者：
Refaeli, Ron [1 ]
Kreisel, Tirzah [1 ]
Goshen, Inbal [1 ]
机构：
[1] Hebrew Univ Jerusalem, Edmond & Lily Safra Ctr Brain Sci ELSC, IL-91904 Jerusalem, Israel
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 01期
基金：
欧洲研究理事会; 以色列科学基金会;
关键词：
Behavior; Microscopy; Model Organisms; Neuroscience;
D O I：
10.1016/j.xpro.2024.102840
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Here, we present a protocol for marking engram cells to efficiently measure reactivation levels and their projection pathways. We describe steps for genetic manipulation utilizing transgenic mice and viral infections, labeling engram cells, and a modified version of CLARITY, a tissue -clearing technique. This protocol can be adapted to various research inquiries that involve assessing the overlap of cell populations and uncovering novel long-range connectivity pathways. For complete details on the use and execution of this protocol, please refer to Refaeli et al. (2023).1
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收藏
页数：17
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