Analyzing engram reactivation and long-range connectivity

被引：0
|
作者：
Refaeli, Ron [1 ]
Kreisel, Tirzah [1 ]
Goshen, Inbal [1 ]
机构：
[1] Hebrew Univ Jerusalem, Edmond & Lily Safra Ctr Brain Sci ELSC, IL-91904 Jerusalem, Israel
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 01期
基金：
欧洲研究理事会; 以色列科学基金会;
关键词：
Behavior; Microscopy; Model Organisms; Neuroscience;
D O I：
10.1016/j.xpro.2024.102840
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Here, we present a protocol for marking engram cells to efficiently measure reactivation levels and their projection pathways. We describe steps for genetic manipulation utilizing transgenic mice and viral infections, labeling engram cells, and a modified version of CLARITY, a tissue -clearing technique. This protocol can be adapted to various research inquiries that involve assessing the overlap of cell populations and uncovering novel long-range connectivity pathways. For complete details on the use and execution of this protocol, please refer to Refaeli et al. (2023).1
引用
收藏
页数：17
相关论文
共 50 条
  • [21] LOCAL AND LONG-RANGE CONNECTIVITY PATTERNS OF AUDITORY PERCEPTUAL DISTURBANCE IN SCHIZOPHRENIA
    Hare, Stephanie
    Adhikari, Bhim
    Chiappelli, Joshua
    Du, Xiaoming
    Chen, Shuo
    Kochunov, Peter
    Hong, L. Elliot
    SCHIZOPHRENIA BULLETIN, 2020, 46 : S3 - S3
  • [22] Effect of synaptic connectivity on long-range synchronization of fast cortical oscillations
    Bazhenov, M.
    Rulkov, N. F.
    Timofeev, I.
    JOURNAL OF NEUROPHYSIOLOGY, 2008, 100 (03) : 1562 - 1575
  • [23] Community-Detection Cellular Automata with Local and Long-Range Connectivity
    Bagnoli, Franco
    Massaro, Emanuele
    Guazzini, Andrea
    CELLULAR AUTOMATA, ACRI 2012, 2012, 7495 : 204 - 213
  • [24] Local versus long-range connectivity patterns of auditory disturbance in schizophrenia
    Hare, Stephanie M.
    Adhikari, Bhim M.
    Du, Xiaoming
    Garcia, Laura
    Bruce, Heather
    Kochunov, Peter
    Simon, Jonathan Z.
    Hong, L. Elliot
    SCHIZOPHRENIA RESEARCH, 2021, 228 : 262 - 270
  • [25] Convergence Speed of the Consensus Algorithm With Interference and Sparse Long-Range Connectivity
    Vanka, Sundaram
    Haenggi, Martin
    Gupta, Vijay
    IEEE JOURNAL OF SELECTED TOPICS IN SIGNAL PROCESSING, 2011, 5 (04) : 855 - 865
  • [26] METHODS FOR ANALYZING TRANSPORT IN DISORDERED-SYSTEMS WITH LONG-RANGE TRAPS
    SHATZ, S
    HALPERN, V
    JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS, 1983, 44 (02) : 113 - 116
  • [27] PyLRO: A Python']Python calculator for analyzing long-range structural order
    Parrish, Kevin
    Hu, Qingyang
    Zhu, Qiang
    JOURNAL OF APPLIED PHYSICS, 2025, 137 (02)
  • [28] Shaping long-range functional connectivity through prefrontal interneuron plasticity
    David A. Kupferschmidt
    Joshua A. Gordon
    Neuropsychopharmacology, 2023, 48 : 240 - 242
  • [29] Analyzing Allostery with Long-Range Rigidity Propagation Across Protein Networks
    Sljoka, Adnan
    BIOPHYSICAL JOURNAL, 2018, 114 (03) : 420A - 420A
  • [30] Statistical Challenges in Analyzing Methylation and Long-Range Chromosomal Interaction Data
    Qin Z.
    Li B.
    Conneely K.N.
    Wu H.
    Hu M.
    Ayyala D.
    Park Y.
    Jin V.X.
    Zhang F.
    Zhang H.
    Li L.
    Lin S.
    Statistics in Biosciences, 2016, 8 (2) : 284 - 309
12345