Analyzing engram reactivation and long-range connectivity

被引：0
|
作者：
Refaeli, Ron [1 ]
Kreisel, Tirzah [1 ]
Goshen, Inbal [1 ]
机构：
[1] Hebrew Univ Jerusalem, Edmond & Lily Safra Ctr Brain Sci ELSC, IL-91904 Jerusalem, Israel
来源：
STAR PROTOCOLS | 2024年 / 5卷 / 01期
基金：
欧洲研究理事会; 以色列科学基金会;
关键词：
Behavior; Microscopy; Model Organisms; Neuroscience;
D O I：
10.1016/j.xpro.2024.102840
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Here, we present a protocol for marking engram cells to efficiently measure reactivation levels and their projection pathways. We describe steps for genetic manipulation utilizing transgenic mice and viral infections, labeling engram cells, and a modified version of CLARITY, a tissue -clearing technique. This protocol can be adapted to various research inquiries that involve assessing the overlap of cell populations and uncovering novel long-range connectivity pathways. For complete details on the use and execution of this protocol, please refer to Refaeli et al. (2023).1
引用
收藏
页数：17
相关论文
共 50 条
  • [31] Multipurpose habitat networks for short-range and long-range connectivity: a new method combining graph and circuit connectivity
    Rayfield, Bronwyn
    Pelletier, David
    Dumitru, Maria
    Cardille, Jeffrey A.
    Gonzalez, Andrew
    METHODS IN ECOLOGY AND EVOLUTION, 2016, 7 (02): : 222 - 231
  • [32] Long-range and very long-range charge transport in DNA
    Bixon, M
    Jortner, J
    CHEMICAL PHYSICS, 2002, 281 (2-3) : 393 - 408
  • [33] Age-related increases in long-range connectivity in fetal functional neural connectivity networks in utero
    Thomason, Moriah E.
    Grove, Lauren E.
    Lozon, Tim A., Jr.
    Vila, Angela M.
    Ye, Yongquan
    Nye, Matthew J.
    Manning, Janessa H.
    Pappas, Athina
    Hernandez-Andrade, Edgar
    Yeo, Lami
    Mody, Swati
    Berman, Susan
    Hassan, Sonia S.
    Romero, Roberto
    DEVELOPMENTAL COGNITIVE NEUROSCIENCE, 2015, 11 : 96 - 104
  • [34] Comprehensive Analysis of Long-Range Connectivity from and to the Posterior Parietal Cortex of the Mouse
    Oh, Seung Wook
    Son, Sook Jin
    Morris, John A.
    Choi, Joon Ho
    Lee, Changkyu
    Rah, Jong-Cheol
    CEREBRAL CORTEX, 2021, 31 (01) : 356 - 378
  • [35] Reduced central white matter volume in autism: Implications for long-range connectivity
    Jou, Roger J.
    Mateljevic, Natasa
    Minshew, Nancy J.
    Keshavan, Matcheri S.
    Hardan, Antonio Y.
    PSYCHIATRY AND CLINICAL NEUROSCIENCES, 2011, 65 (01) : 98 - 101
  • [36] The role of long-range connectivity for the characterization of the functional-anatomical organization of the cortex
    Knosche, Thomas R.
    Tittgemeyer, Marc
    FRONTIERS IN SYSTEMS NEUROSCIENCE, 2011, 5
  • [37] Chromatin connectivity maps reveal dynamic promoter–enhancer long-range associations
    Yubo Zhang
    Chee-Hong Wong
    Ramon Y. Birnbaum
    Guoliang Li
    Rebecca Favaro
    Chew Yee Ngan
    Joanne Lim
    Eunice Tai
    Huay Mei Poh
    Eleanor Wong
    Fabianus Hendriyan Mulawadi
    Wing-Kin Sung
    Silvia Nicolis
    Nadav Ahituv
    Yijun Ruan
    Chia-Lin Wei
    Nature, 2013, 504 : 306 - 310
  • [38] Abnormal local and long-range functional connectivity in schizophrenia during ultimatum game
    Yang-Tae, K.
    Kyongsik, Y.
    Jaeseung, J.
    EUROPEAN NEUROPSYCHOPHARMACOLOGY, 2011, 21 : S454 - S455
  • [39] Reduced Central White Matter Volume in Autism: Implications for Long-Range Connectivity
    Jou, Roger J.
    Hardan, Antonio Y.
    BIOLOGICAL PSYCHIATRY, 2009, 65 (08) : 87S - 88S
  • [40] Reduced long-range functional connectivity in young children with autism spectrum disorder
    Kikuchi, Mitsuru
    Yoshimura, Yuko
    Hiraishi, Hirotoshi
    Munesue, Toshio
    Hashimoto, Takanori
    Tsubokawa, Tsunehisa
    Takahashi, Tsutomu
    Suzuki, Michio
    Higashida, Haruhiro
    Minabe, Yoshio
    SOCIAL COGNITIVE AND AFFECTIVE NEUROSCIENCE, 2015, 10 (02) : 248 - 254
12345