Kinetic measurements of membrane target level and on-cell binding of biotherapeutic antibodies using flow-cytometry

被引：0
|
作者：
Daniel T. Doty [1 ]
Mahua Roy [2 ]
Phanidhar Kukutla [3 ]
Nicholas Lucarelli [1 ]
Peter E. Struss [6 ]
Lindsay E. King [1 ]
Jeffrey R. Chabot [4 ]
Jatin Narula [1 ]
Hendrik Neubert [5 ]
Polina Goihberg [1 ]
机构：
[1] Pfizer Research and Development,Biomarkers and Biomeasures, Department of Pharmacokinetics, Dynamics and Metabolism
[2] Department of Pharmacokinetics,Translational Modeling and Simulation
[3] Dynamics and Metabolism,Present Address: College of Medicine, Department of Medicine
[4] Pfizer Research and Development,Present Address: Clinical and Translational Biomarkers, Translational Clinical Sciences
[5] Present Address: Clinical Pharmacology and Quantitative Pharmacology,undefined
[6] AstraZeneca,undefined
[7] University of Florida,undefined
[8] Present Address: BioLegend,undefined
[9] Present Address: Thinkhat Venture Studios Inc.,undefined
[10] Pfizer Research and Development,undefined
来源：
AAPS Open | / 11卷 / 1期
关键词：
Antibodies; Monoclonal; Mathematical models; Biotherapeutics; Target expression; Drug discovery;
D O I：
10.1186/s41120-025-00112-8
中图分类号：
学科分类号：
摘要：
引用
收藏
相关论文
共 50 条
  • [21] KINETIC-ANALYSIS OF CYTOTOXIC LYMPHOCYTE TARGET-CELL INTERACTION AS QUANTIFIED BY DUAL PARAMETER FLOW-CYTOMETRY
    WANIEWSKI, J
    PALUCKA, AK
    PORWIT, A
    CYTOMETRY, 1993, 14 (04): : 393 - 400
  • [22] DETECTION OF ANTISPERM ANTIBODIES BY IMMOBILIZATION AND CYTOTOXICITY TESTS USING FLOW-CYTOMETRY
    NIKOLAEVA, MN
    VERBITSKII, MS
    SUKHIKH, GT
    BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 1992, 114 (10) : 1491 - 1493
  • [23] A RAPID, OBJECTIVE METHOD FOR THE DETECTION OF LYMPHOCYTOTOXIC ANTIBODIES USING FLOW-CYTOMETRY
    TALBOT, D
    SHENTON, BK
    GIVAN, AL
    PROUD, G
    TAYLOR, RMR
    JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 99 (01) : 137 - 140
  • [24] IDENTIFICATION OF CELLS IN MITOSIS BY FLOW-CYTOMETRY USING MONOCLONAL-ANTIBODIES
    DAVIS, FM
    PERSHOUSE, M
    BARLOGIE, B
    RAO, PN
    PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, 1985, 26 (MAR): : 29 - 29
  • [25] ANALYSIS OF PLATELET ANTIGENS FOR ANTIPLATETLET ANTIBODIES IN ITP USING FLOW-CYTOMETRY
    NOMURA, S
    NAGATA, H
    SONE, N
    ODA, K
    KOKAWA, T
    YASUNAGA, K
    THROMBOSIS AND HAEMOSTASIS, 1987, 58 (01) : 481 - 481
  • [26] THE STUDY OF THE EFFECTS OF MURINE ANTICARDIOLIPIN MONOCLONAL-ANTIBODIES ON BINDING OF HUMAN ANTICARDIOLIPIN ANTIBODIES BY FLOW-CYTOMETRY
    CHEN, JC
    POULIK, MD
    FASEB JOURNAL, 1993, 7 (03): : A55 - A55
  • [27] A COMPARISON OF IMMUNOBLOTTING, FLOW-CYTOMETRY AND ELISA TO MONITOR THE BINDING OF ANTILIPOPOLYSACCHARIDE MONOCLONAL-ANTIBODIES
    NELSON, D
    NEILL, W
    POXTON, IR
    JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 133 (02) : 227 - 233
  • [28] ANALYSIS OF HUMAN-LEUKEMIC CELL-POPULATIONS BY FLOW-CYTOMETRY USING MONOCLONAL-ANTIBODIES
    ZOLA, H
    BRADLEY, J
    BECKMAN, IGR
    BROOKS, DA
    MCNAMARA, PJ
    CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 1981, 8 (04) : 384 - 384
  • [29] CELL KINETIC-ANALYSIS OF MIXED POPULATIONS USING 3-COLOR FLUORESCENCE FLOW-CYTOMETRY
    BEGG, AC
    HOFLAND, I
    CYTOMETRY, 1991, 12 (05): : 445 - 454
  • [30] MEASUREMENT OF CYTOPLASMIC CALCIUM IN LYMPHOCYTES USING FLOW-CYTOMETRY - KINETIC-STUDIES AND SINGLE CELL ANALYSIS
    GRIFFIOEN, AW
    RIJKERS, GT
    KEIJ, J
    ZEGERS, BJM
    JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 120 (01) : 23 - 27
12345