DNA breakpoints and free DNA fragments as potential predictors of infertility

被引：0
|
作者：
Zhou, Yue [1 ]
Wang, Juan [1 ,4 ]
Zhang, Fan [2 ]
Pei, Liguo [1 ]
Chang, Yue [4 ]
Yan, Bei [1 ,5 ]
Wang, Hongyan [1 ,3 ]
机构：
[1] Ningxia Med Univ, Gen Hosp, Inst Med Sci, Key Lab Fertil Preservat & Maintenance,Minist Edu, Yinchuan 750004, Peoples R China
[2] Yinchuan Women & Children Healthcare Hosp, Reprod Ctr, Yinchuan 750004, Peoples R China
[3] South China Univ Technol, Sch Med, Div Cell Dev & Integrat Biol, Guangzhou 510006, Peoples R China
[4] Ningxia Med Univ, Dept Pathol, Yinchuan 750004, Peoples R China
[5] Huazhong Univ Sci & Technol, Inst Reprod Hlth, Tongji Med Coll, Sch Pharm, Wuhan 430000, Peoples R China
来源：
SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期
关键词：
Varicocele; Sperm analysis; Mean number of DNA breakpoint; Free DNA in seminal plasma; ROS; MEAN NUMBER; SPERM; VARICOCELE; DAMAGE; ASSAY;
D O I：
10.1038/s41598-024-81252-x
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The study investigated the impact of varicocele on male infertility by analyzing sperm samples from 42 varicocele patients and 22 healthy individuals. Basic sperm parameters such as concentration and vitality were assessed, along with the DNA Fragmentation Index (DFI) using the SCSA method. Additionally, the mean number of sperm DNA breaks (MDB) and free DNA fragments amount in seminal plasma (fDFA) were analyzed using the TDT-Strand Displacement Probe Technique. Results showed higher levels of DFI, MDB, and fDFA in varicocele-related infertility cases. Combining MDB and fDFA proved more effective in predicting fertility compared to individual assessments or the DFI alone. While these findings suggest a potential role for MDB and fDFA as predictors of male fertility, further rigorous studies are needed to validate their application in varicocele-related male infertility.
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页数：9
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