DNA breakpoints and free DNA fragments as potential predictors of infertility

被引：0

作者：

Zhou, Yue ^{[1
]}

Wang, Juan ^{[1
,4
]}

Zhang, Fan ^{[2
]}

Pei, Liguo ^{[1
]}

Chang, Yue ^{[4
]}

Yan, Bei ^{[1
,5
]}

Wang, Hongyan ^{[1
,3
]}

机构：

[1] Ningxia Med Univ, Gen Hosp, Inst Med Sci, Key Lab Fertil Preservat & Maintenance,Minist Edu, Yinchuan 750004, Peoples R China

[2] Yinchuan Women & Children Healthcare Hosp, Reprod Ctr, Yinchuan 750004, Peoples R China

[3] South China Univ Technol, Sch Med, Div Cell Dev & Integrat Biol, Guangzhou 510006, Peoples R China

[4] Ningxia Med Univ, Dept Pathol, Yinchuan 750004, Peoples R China

[5] Huazhong Univ Sci & Technol, Inst Reprod Hlth, Tongji Med Coll, Sch Pharm, Wuhan 430000, Peoples R China

来源：

SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期

关键词：

Varicocele; Sperm analysis; Mean number of DNA breakpoint; Free DNA in seminal plasma; ROS; MEAN NUMBER; SPERM; VARICOCELE; DAMAGE; ASSAY;

D O I：

10.1038/s41598-024-81252-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The study investigated the impact of varicocele on male infertility by analyzing sperm samples from 42 varicocele patients and 22 healthy individuals. Basic sperm parameters such as concentration and vitality were assessed, along with the DNA Fragmentation Index (DFI) using the SCSA method. Additionally, the mean number of sperm DNA breaks (MDB) and free DNA fragments amount in seminal plasma (fDFA) were analyzed using the TDT-Strand Displacement Probe Technique. Results showed higher levels of DFI, MDB, and fDFA in varicocele-related infertility cases. Combining MDB and fDFA proved more effective in predicting fertility compared to individual assessments or the DFI alone. While these findings suggest a potential role for MDB and fDFA as predictors of male fertility, further rigorous studies are needed to validate their application in varicocele-related male infertility.

引用

页数：9

共 50 条

[11] A DNA Methylation Perspective on Infertility
Shacfe, Ghaleb
Turko, Rasoul
Syed, Haadi Hammad
Masoud, Ibraheem
Tahmaz, Yahya
Samhan, Lara M.
Alkattan, Khaled
Shafqat, Areez
Yaqinuddin, Ahmed
GENES, 2023, 14 (12)
[12] DNA methylation and male infertility
Cisneros, FJ
FRONTIERS IN BIOSCIENCE-LANDMARK, 2004, 9 : 1189 - 1200
[13] THE KU AUTOANTIGEN INHIBITS TRANSCRIPTION OF FREE-END DNA FRAGMENTS
ONO, M
TUCKER, PW
SONTHEIMER, RD
UEKI, H
CAPRA, JD
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1995, 104 (04) : 674 - 674
[14] Mean number of DNA breakpoints: illuminating sperm DNA integrity and in vitro fertilization outcomes
Zhou, Wenjing
Zhang, Jianeng
Cheng, Zhaojun
Wang, Chong
Feng, Ying
FERTILITY AND STERILITY, 2024, 121 (02) : 264 - 270
[15] Spermatogenesis, DNA damage and DNA repair mechanisms in male infertility
Gunes, Sezgin
Al-Sadaan, Maha
Agarwal, Ashok
REPRODUCTIVE BIOMEDICINE ONLINE, 2015, 31 (03) : 309 - 319
[16] CIRCULATING FREE GENOMIC AND MITOCHONDRIAL DNA FRAGMENTS AND THEIR DIAGNOSTIC AND PROGNOSTIC POTENTIAL IN CLEAR CELL RENAL CELL CARCINOMA PATIENTS
Ralla, Bernhard
Hongbiao, Luis
Jung, Monika
Ergin, Kilic
Budach, Nils
Fendler, Annika
Jung, Klaus
Busch, Jonas
JOURNAL OF UROLOGY, 2016, 195 (04): : E1104 - E1105
[17] Duplication and triplication with staggered breakpoints in human mitochondrial DNA
Tengan, C. H.
Moraes, C. T.
B B A - Biomembranes, 1406 (01):
[18] Duplication and triplication with staggered breakpoints in human mitochondrial DNA
Tengan, CH
Moraes, CT
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1998, 1406 (01): : 73 - 80
[19] ANALYSIS OF LARGE DNA FRAGMENTS
WEISS, AS
SEARCH, 1987, 18 (06): : 302 - 303
[20] Banks of large DNA fragments
Rogel-Gaillard, C
PRODUCTIONS ANIMALES, 2000, : 79 - 85

← 1 2 3 4 5 →