DNA breakpoints and free DNA fragments as potential predictors of infertility

被引：0
|
作者：
Zhou, Yue [1 ]
Wang, Juan [1 ,4 ]
Zhang, Fan [2 ]
Pei, Liguo [1 ]
Chang, Yue [4 ]
Yan, Bei [1 ,5 ]
Wang, Hongyan [1 ,3 ]
机构：
[1] Ningxia Med Univ, Gen Hosp, Inst Med Sci, Key Lab Fertil Preservat & Maintenance,Minist Edu, Yinchuan 750004, Peoples R China
[2] Yinchuan Women & Children Healthcare Hosp, Reprod Ctr, Yinchuan 750004, Peoples R China
[3] South China Univ Technol, Sch Med, Div Cell Dev & Integrat Biol, Guangzhou 510006, Peoples R China
[4] Ningxia Med Univ, Dept Pathol, Yinchuan 750004, Peoples R China
[5] Huazhong Univ Sci & Technol, Inst Reprod Hlth, Tongji Med Coll, Sch Pharm, Wuhan 430000, Peoples R China
来源：
SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期
关键词：
Varicocele; Sperm analysis; Mean number of DNA breakpoint; Free DNA in seminal plasma; ROS; MEAN NUMBER; SPERM; VARICOCELE; DAMAGE; ASSAY;
D O I：
10.1038/s41598-024-81252-x
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The study investigated the impact of varicocele on male infertility by analyzing sperm samples from 42 varicocele patients and 22 healthy individuals. Basic sperm parameters such as concentration and vitality were assessed, along with the DNA Fragmentation Index (DFI) using the SCSA method. Additionally, the mean number of sperm DNA breaks (MDB) and free DNA fragments amount in seminal plasma (fDFA) were analyzed using the TDT-Strand Displacement Probe Technique. Results showed higher levels of DFI, MDB, and fDFA in varicocele-related infertility cases. Combining MDB and fDFA proved more effective in predicting fertility compared to individual assessments or the DFI alone. While these findings suggest a potential role for MDB and fDFA as predictors of male fertility, further rigorous studies are needed to validate their application in varicocele-related male infertility.
引用
收藏
页数：9
相关论文
共 50 条
  • [31] MLL genomic DNA Breakpoints In Infant Acute Leukemia
    Tsaur, Grigory
    Meyer, Claus
    Popov, Alexander
    Plekhanova, Olga
    Kustanovich, Anatoly
    Volochnik, Alena
    Riger, Tatyana
    Demina, Anna
    Druy, Alexander
    Fleischman, Elena
    Sokova, Olga
    Olshanskaya, Yulia
    Streneva, Olga
    Shorikov, Egor
    Saveliev, Leonid
    Marschalek, Rolf
    Fechima, Larisa
    BLOOD, 2013, 122 (21)
  • [32] Tracking genome engineering outcome at individual DNA breakpoints
    Certo, Michael T.
    Ryu, Byoung Y.
    Annis, James E.
    Garibov, Mikhail
    Jarjour, Jordan
    Rawlings, David J.
    Scharenberg, Andrew M.
    NATURE METHODS, 2011, 8 (08) : 671 - U102
  • [33] Tracking genome engineering outcome at individual DNA breakpoints
    Certo M.T.
    Ryu B.Y.
    Annis J.E.
    Garibov M.
    Jarjour J.
    Rawlings D.J.
    Scharenberg A.M.
    Nature Methods, 2011, 8 (8) : 671 - 676
  • [34] Translocation Breakpoints and G-quadruplex DNA.
    Williams, J.
    Fleetwood, S.
    Larson, E.
    ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2014, 55 : S53 - S53
  • [35] The application of mean number of DNA breakpoints in sperm cryopreservation
    Yan, Bei
    Wang, Juan
    Zhou, Yue
    Pei, Liguo
    Zhang, Fan
    Gao, Bianbian
    Wang, Hongyan
    CRYOBIOLOGY, 2024, 116
  • [36] DNA Probe Pooling for Rapid Delineation of Chromosomal Breakpoints
    Lu, Chun-Mei
    Kwan, Johnson
    Baumgartner, Adolf
    Weier, Jingly F.
    Wang, Mei
    Escudero, Tomas
    Munne, Santiago
    Zitzelsberger, Horst F.
    Weier, Heinz-Ulrich G.
    JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2009, 57 (06) : 587 - 597
  • [37] Cell free DNA as potential marker of inflammatory diseases
    Smolak, D.
    Roman, G.
    Budis, J.
    Maronek, M.
    Gromova, B.
    Liptak, R.
    Harsanyova, M.
    Radvanszky, J.
    Szemes, T.
    EUROPEAN JOURNAL OF HUMAN GENETICS, 2020, 28 (SUPPL 1) : 230 - 231
  • [38] Cell-free DNA as a potential biomarker for preeclampsia
    Palei, Ana C.
    EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2021, 21 (12) : 1253 - 1256
  • [39] Cell-Free DNA in Rhabdomyosarcoma: Unravelling The Potential
    Lak, N.
    Van Zogchel, L.
    Zappeij-Kannegieter, L.
    Javadi, A.
    Van Paemel, R.
    Vandeputte, C.
    De Preter, K.
    De Wilde, B.
    Chicard, M.
    Schleiermacher, G.
    Ruhen, O.
    Shipley, J.
    Merks, J.
    Van Noesel, M. M.
    Van der Schoot, C. E.
    Tytgat, L.
    Stutterheim, J.
    PEDIATRIC BLOOD & CANCER, 2021, 68 : S259 - S259
  • [40] Enrichment of fetal and maternal long cell-free DNA fragments from maternal plasma following DNA repair
    Vong, Joaquim S. L.
    Jiang, Peiyong
    Cheng, Suk-Hang
    Lee, Wing-Shan
    Tsang, Jason C. H.
    Leung, Tak-Yeung
    Chan, K. C. Allen
    Chiu, Rossa W. K.
    Lo, Y. M. Dennis
    PRENATAL DIAGNOSIS, 2019, 39 (02) : 88 - 99
12345