Highly sequence-specific, timing-controllable m6A demethylation by modulating RNA-binding affinity of m6A erasers

被引：0

作者：

Otonari, Kenko ^{[1
]}

Asami, Yuri ^{[1
]}

Ogata, Kosuke ^{[3
]}

Ishihama, Yasushi ^{[2
,3
]}

Futaki, Shiroh ^{[1
]}

Imanishi, Miki ^{[1
]}

机构：

[1] Kyoto Univ, Inst Chem Res, Uji, Kyoto 6110011, Japan

[2] Kyoto Univ, Grad Sch Pharmaceut Sci, Kyoto 6068501, Japan

[3] Natl Inst Biomed Innovat Hlth & Nutr, Ibaraki, Osaka 5670085, Japan

来源：

CHEMICAL COMMUNICATIONS | 2024年 / 61卷 / 01期

关键词：

NUCLEAR-RNA; SUBSTRATE; ALKBH5;

D O I：

10.1039/d4cc04070h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Recent advancements in tools using programmable RNA binding proteins and m(6)A-erasers enable sequence-selective and timing-controllable m(6)A demethylation. However, off-target effects are still a concern. This study addresses the problem by reducing the RNA-binding ability of m(6)A-erasers. The modulated m(6)A-erasers achieved sequence-specific and timing-controllable m(6)A demethylation with minimal off-target activity.

引用

页码：69 / 72

页数：5

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