Highly sequence-specific, timing-controllable m6A demethylation by modulating RNA-binding affinity of m6A erasers

被引：0
|
作者：
Otonari, Kenko [1 ]
Asami, Yuri [1 ]
Ogata, Kosuke [3 ]
Ishihama, Yasushi [2 ,3 ]
Futaki, Shiroh [1 ]
Imanishi, Miki [1 ]
机构：
[1] Kyoto Univ, Inst Chem Res, Uji, Kyoto 6110011, Japan
[2] Kyoto Univ, Grad Sch Pharmaceut Sci, Kyoto 6068501, Japan
[3] Natl Inst Biomed Innovat Hlth & Nutr, Ibaraki, Osaka 5670085, Japan
来源：
CHEMICAL COMMUNICATIONS | 2024年 / 61卷 / 01期
关键词：
NUCLEAR-RNA; SUBSTRATE; ALKBH5;
D O I：
10.1039/d4cc04070h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Recent advancements in tools using programmable RNA binding proteins and m(6)A-erasers enable sequence-selective and timing-controllable m(6)A demethylation. However, off-target effects are still a concern. This study addresses the problem by reducing the RNA-binding ability of m(6)A-erasers. The modulated m(6)A-erasers achieved sequence-specific and timing-controllable m(6)A demethylation with minimal off-target activity.
引用
收藏
页码：69 / 72
页数：5
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