Piezoelectric urea biosensor based on immobilization of urease onto nanoporous alumina membranes

被引：0
|
作者：
College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China [1 ]
机构：
来源：
Biosens. Bioelectron. | 2007年 / 12卷 / 3283-3287期
基金：
中国国家自然科学基金;
关键词：
Alumina - Enzyme immobilization - Membranes - Nanopores - Piezoelectric devices - Urea;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The urease was immobilized onto nanoporous alumina membranes prepared by the two-step anodization method, and a novel piezoelectric urea sensing system with separated porous alumina/urease electrode has been developed through measuring the conductivity change of immobilized urease/urea reaction. The process of urease immobilization was optimized and the performance of the developed urea biosensor was evaluated. The obtained urea biosensor presented high-selectivity monitoring of urea, better reproducibility (S.D. = 0.02, n = 6), shorter response time (30 s), wider linear range (0.5 μM to 3 mM), lower detection limit (0.2 μM) and good long-term storage stability (with about 76% of the enzymatic activity retained after 30 days). The clinical analysis of the urea biosensor confirmed the feasibility of urea detection in urine samples. © 2007 Elsevier B.V. All rights reserved.
引用
收藏
相关论文
共 50 条
  • [31] New Urea Biosensor Based on Urease Enzyme Obtained from Helycobacter pylori
    Bahar Dindar
    Emine Karakuş
    Fatih Abasıyanık
    Applied Biochemistry and Biotechnology, 2011, 165 : 1308 - 1321
  • [32] Urea determination in FIA mode by a newly designed urease-based biosensor
    Milardovic, S
    Kruhak, I
    Grabaric, BS
    LABORATORY ROBOTICS AND AUTOMATION, 1999, 11 (05) : 266 - 271
  • [33] Urea potentiometric biosensor based on modified electrodes with urease immobilized on polyethylenimine films
    Lakard, B
    Herlem, G
    Lakard, S
    Antoniou, A
    Fahys, B
    BIOSENSORS & BIOELECTRONICS, 2004, 19 (12): : 1641 - 1647
  • [34] Urea biosensor based on the composite film of electroinactive polypyrrole and urease modified with polyanion
    Komaba, S
    Seyama, M
    Tanabe, K
    Osaka, T
    DENKI KAGAKU, 1996, 64 (12): : 1228 - 1233
  • [35] Microfabricated conductometric urea biosensor based on sol-gel immobilized urease
    Lee, WY
    Lee, KS
    Kim, TH
    Shin, MC
    Park, JK
    ELECTROANALYSIS, 2000, 12 (01) : 78 - 82
  • [36] POLYPYRROLE-BASED POTENTIOMETRIC BIOSENSOR FOR UREA .1. INCORPORATION OF UREASE
    ADELOJU, SB
    SHAW, SJ
    WALLACE, GG
    ANALYTICA CHIMICA ACTA, 1993, 281 (03) : 611 - 620
  • [37] Nanoporous Alumina Membranes Based Microdevices for Ultrasensitive Protein Detection
    Bothara, M. G.
    Reddy, R. K.
    Barrett, T.
    Carruthers, J.
    Prasad, S.
    NSTI NANOTECH 2008, VOL 1, TECHNICAL PROCEEDINGS: MATERIALS, FABRICATION, PARTICLES, AND CHARACTERIZATION, 2008, : 344 - 347
  • [38] Thermal radiation shielding by nanoporous membranes based on anodic alumina
    Muratova, E. N.
    Matyushkin, L. B.
    Moshnikov, V. A.
    Chernyakova, K. V.
    Vrublevsky, I. A.
    24TH INTERNATIONAL CONFERENCE ON VACUUM TECHNIQUE AND TECHNOLOGY, 2017, 872
  • [39] Fullerene-cryptand coated piezoelectric crystal urea sensor based on urease
    Wei, LF
    Shih, JS
    ANALYTICA CHIMICA ACTA, 2001, 437 (01) : 77 - 85
  • [40] Urea impedimetric biosensor based on polymer degradation onto interdigitated electrodes
    Cortina, M.
    Esplandiu, M. J.
    Alegret, S.
    del Valle, M.
    SENSORS AND ACTUATORS B-CHEMICAL, 2006, 118 (1-2) : 84 - 89
12345