Piezoelectric urea biosensor based on immobilization of urease onto nanoporous alumina membranes

被引：0
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作者：
College of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, China [1 ]
机构：
来源：
Biosens. Bioelectron. | 2007年 / 12卷 / 3283-3287期
基金：
中国国家自然科学基金;
关键词：
Alumina - Enzyme immobilization - Membranes - Nanopores - Piezoelectric devices - Urea;
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摘要：
The urease was immobilized onto nanoporous alumina membranes prepared by the two-step anodization method, and a novel piezoelectric urea sensing system with separated porous alumina/urease electrode has been developed through measuring the conductivity change of immobilized urease/urea reaction. The process of urease immobilization was optimized and the performance of the developed urea biosensor was evaluated. The obtained urea biosensor presented high-selectivity monitoring of urea, better reproducibility (S.D. = 0.02, n = 6), shorter response time (30 s), wider linear range (0.5 μM to 3 mM), lower detection limit (0.2 μM) and good long-term storage stability (with about 76% of the enzymatic activity retained after 30 days). The clinical analysis of the urea biosensor confirmed the feasibility of urea detection in urine samples. © 2007 Elsevier B.V. All rights reserved.
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