POLYPYRROLE-BASED POTENTIOMETRIC BIOSENSOR FOR UREA .1. INCORPORATION OF UREASE

被引：83

作者：

ADELOJU, SB ^{[1
]}

SHAW, SJ ^{[1
]}

WALLACE, GG ^{[1
]}

机构：

[1] UNIV WOLLONGONG,DEPT CHEM,INTELLIGENT POLYMER RES LAB,WOLLONGONG,NSW 2500,AUSTRALIA

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 281卷 / 03期

关键词：

BIOSENSORS; POTENTIOMETRY; ENZYMATIC METHODS; CONDUCTING POLYMER; POLYPYRROLE; UREA; UREASE;

D O I：

10.1016/0003-2670(93)85022-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is described for the incorporation of urease into a polypyrrole film by galvanostatic polymerisation on gold-coated plastic films and solid gold electrodes. The presence of urease in the polypyrrole film was verified by x-ray photoelectron spectroscopy, amino acid analysis, scanning electron microscopy and cyclic voltammetry. The enzyme activity in the polypyrrole-urease film was confirmed by its catalytic conversion of urea to carbon dioxide and ammonia. The use of both spectrophotometric and potentiometric methods for the detection of the catalytic activity of the polypyrrole-urease film and their potential use for the determination of urea is discussed.

引用

页码：611 / 620

页数：10

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