Evaluation of the Humoral Response after Immunization with a Chimeric Subunit Vaccine against Shiga Toxin-Producing Escherichia coli in Pregnant Sows and Their Offspring
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作者:
Vidal, Roberto M.
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Univ Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
Univ Chile, Fac Med, Inst Milenio Inmunol & Inmunoterapia, Santiago 8380453, ChileUniv Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
Vidal, Roberto M.
[1
,2
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Montero, David A.
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Univ Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
Univ Bernardo OHiggins, Ctr Integrat Biol & Quim Aplicada CIBQA, Santiago 8320000, ChileUniv Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
Montero, David A.
[1
,3
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Bentancor, Adriana
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机构:
Univ Buenos Aires, Fac Ciencias Vet, Inst Invest Epidemiol Vet, Catedra Microbiol, C1427CWO, Buenos Aires, ArgentinaUniv Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
Bentancor, Adriana
[4
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Arellano, Carolina
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Univ Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, ChileUniv Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
Arellano, Carolina
[1
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Alvarez, Alhejandra
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Univ Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, ChileUniv Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
Alvarez, Alhejandra
[1
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Cundon, Cecilia
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机构:
Univ Buenos Aires, Fac Ciencias Vet, Inst Invest Epidemiol Vet, Catedra Microbiol, C1427CWO, Buenos Aires, ArgentinaUniv Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
Cundon, Cecilia
[4
]
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Crivelli, Ximena Blanco
[4
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Del Canto, Felipe
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Univ Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, ChileUniv Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
Del Canto, Felipe
[1
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Salazar, Juan C.
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Univ Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, ChileUniv Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
Salazar, Juan C.
[1
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Onate, Angel A.
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机构:
Univ Concepcion, Fac Ciencias Biol, Dept Microbiol, Concepcion 4070386, ChileUniv Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
Onate, Angel A.
[5
]
机构:
[1] Univ Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
[2] Univ Chile, Fac Med, Inst Milenio Inmunol & Inmunoterapia, Santiago 8380453, Chile
Shiga toxin-producing Escherichia coli (STEC) poses a significant public health risk due to its zoonotic potential and association with severe human diseases, such as hemorrhagic colitis and hemolytic uremic syndrome. Ruminants are recognized as primary reservoirs for STEC, but swine also contribute to the epidemiology of this pathogen, highlighting the need for effective prevention strategies across species. Notably, a subgroup of STEC that produces Shiga toxin type 2e (Stx2e) causes edema disease (ED) in newborn piglets, economically affecting pig production. This study evaluates the immunogenicity of a chimeric protein-based vaccine candidate against STEC in pregnant sows and the subsequent transfer of immunity to their offspring. This vaccine candidate, which includes chimeric proteins displaying selected epitopes from the proteins Cah, OmpT, and Hes, was previously proven to be immunogenic in pregnant cows. Our analysis revealed a broad diversity of STEC serotypes within swine populations, with the cah and ompT genes being prevalent, validating them as suitable antigens for vaccine development. Although the hes gene was detected less frequently, the presence of at least one of these three genes in a significant proportion of STEC suggests the potential of this vaccine to target a wide range of strains. The vaccination of pregnant sows led to an increase in specific IgG and IgA antibodies against the chimeric proteins, indicating successful immunization. Additionally, our results demonstrated the effective passive transfer of maternal antibodies to piglets, providing them with immediate, albeit temporary, humoral immunity against STEC. These humoral responses demonstrate the immunogenicity of the vaccine candidate and are preliminary indicators of its potential efficacy. However, further research is needed to conclusively evaluate its impact on STEC colonization and shedding. This study highlights the potential of maternal vaccination to protect piglets from ED and contributes to the development of vaccination strategies to reduce the prevalence of STEC in various animal reservoirs.
机构:
ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USAARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA
Kalchayanand, Norasak
Koohmaraie, Mohammad
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机构:
IEH Labs & Consulting Grp, 15300 Bothell Way NE, Lake Forest Pk, WA 98155 USAARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA
Koohmaraie, Mohammad
Wheeler, Tommy L.
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机构:
ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USAARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA
机构:
Guilan Univ Med Sci, Rasht, IranZabol Univ Med Sci, Zabol Med Plants Res Ctr, Zabol, Iran
Soufi, L.
Ayatollahi, S. A. M.
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机构:
Shahid Beheshti Univ Med Sci, Phytochem Res Ctr, Tehran, Iran
Shahid Beheshti Univ Med Sci, Sch Pharm, Dept Pharmacognosy, Tehran, IranZabol Univ Med Sci, Zabol Med Plants Res Ctr, Zabol, Iran
Ayatollahi, S. A. M.
Iriti, M.
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Milan State Univ, Dept Agr & Environm Sci, Milan, ItalyZabol Univ Med Sci, Zabol Med Plants Res Ctr, Zabol, Iran
Iriti, M.
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Sharifi-Rad, M.
Varoni, E. M.
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Milan State Univ, Dept Biomed Surg & Dent Sci, Milan, ItalyZabol Univ Med Sci, Zabol Med Plants Res Ctr, Zabol, Iran
Varoni, E. M.
Shahri, F.
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机构:
Zahedan Univ Med Sci, Sch Rehabil, Dept Optometry, Zahedan, IranZabol Univ Med Sci, Zabol Med Plants Res Ctr, Zabol, Iran
Shahri, F.
Esposito, S.
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机构:
Univ Milan, Fdn IRCCS Ca Granda Osped Maggiore Policlin, Dept Pathophysiol & Transplantat, Pediat High Intens Care Unit, Milan, ItalyZabol Univ Med Sci, Zabol Med Plants Res Ctr, Zabol, Iran
Esposito, S.
Kuhestani, K.
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Zabol Univ Med Sci, Zabol, IranZabol Univ Med Sci, Zabol Med Plants Res Ctr, Zabol, Iran
机构:
Jeonbuk Natl Univ, Coll Vet Med, 79 Gobong Ro, Iksan Si 56443, Jeollabuk Do, South KoreaJeonbuk Natl Univ, Coll Vet Med, 79 Gobong Ro, Iksan Si 56443, Jeollabuk Do, South Korea
机构:
Univ Urbino Carlo Bo, Dipartimento Sci Biomol, Urbino, PU, Italy
USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USAUniv Urbino Carlo Bo, Dipartimento Sci Biomol, Urbino, PU, Italy
Rotundo, Luca
Carloni, Elisa
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Univ Urbino Carlo Bo, Dipartimento Sci Biomol, Urbino, PU, ItalyUniv Urbino Carlo Bo, Dipartimento Sci Biomol, Urbino, PU, Italy
Carloni, Elisa
Omiccioli, Enrica
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Diatheva Srl, Fano, PU, ItalyUniv Urbino Carlo Bo, Dipartimento Sci Biomol, Urbino, PU, Italy
Omiccioli, Enrica
Magnani, Mauro
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Univ Urbino Carlo Bo, Dipartimento Sci Biomol, Urbino, PU, ItalyUniv Urbino Carlo Bo, Dipartimento Sci Biomol, Urbino, PU, Italy
Magnani, Mauro
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Brandi, Giorgio
Fratamico, Pina
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USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USAUniv Urbino Carlo Bo, Dipartimento Sci Biomol, Urbino, PU, Italy