Evaluation of the Humoral Response after Immunization with a Chimeric Subunit Vaccine against Shiga Toxin-Producing Escherichia coli in Pregnant Sows and Their Offspring

被引:0
|
作者
Vidal, Roberto M. [1 ,2 ]
Montero, David A. [1 ,3 ]
Bentancor, Adriana [4 ]
Arellano, Carolina [1 ]
Alvarez, Alhejandra [1 ]
Cundon, Cecilia [4 ]
Crivelli, Ximena Blanco [4 ]
Del Canto, Felipe [1 ]
Salazar, Juan C. [1 ]
Onate, Angel A. [5 ]
机构
[1] Univ Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed, Santiago 8380453, Chile
[2] Univ Chile, Fac Med, Inst Milenio Inmunol & Inmunoterapia, Santiago 8380453, Chile
[3] Univ Bernardo OHiggins, Ctr Integrat Biol & Quim Aplicada CIBQA, Santiago 8320000, Chile
[4] Univ Buenos Aires, Fac Ciencias Vet, Inst Invest Epidemiol Vet, Catedra Microbiol, C1427CWO, Buenos Aires, Argentina
[5] Univ Concepcion, Fac Ciencias Biol, Dept Microbiol, Concepcion 4070386, Chile
关键词
Shiga toxin-producing Escherichia coli; STEC; chimeric protein based-vaccine; edema disease of swine; pigs; EDEMA DISEASE; WEANED PIGLETS; COLONIZATION; PREVALENCE; FIMBRIAE; SWINE; PIGS; IGG; PROTECTION; COMPONENTS;
D O I
10.3390/vaccines12070726
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Shiga toxin-producing Escherichia coli (STEC) poses a significant public health risk due to its zoonotic potential and association with severe human diseases, such as hemorrhagic colitis and hemolytic uremic syndrome. Ruminants are recognized as primary reservoirs for STEC, but swine also contribute to the epidemiology of this pathogen, highlighting the need for effective prevention strategies across species. Notably, a subgroup of STEC that produces Shiga toxin type 2e (Stx2e) causes edema disease (ED) in newborn piglets, economically affecting pig production. This study evaluates the immunogenicity of a chimeric protein-based vaccine candidate against STEC in pregnant sows and the subsequent transfer of immunity to their offspring. This vaccine candidate, which includes chimeric proteins displaying selected epitopes from the proteins Cah, OmpT, and Hes, was previously proven to be immunogenic in pregnant cows. Our analysis revealed a broad diversity of STEC serotypes within swine populations, with the cah and ompT genes being prevalent, validating them as suitable antigens for vaccine development. Although the hes gene was detected less frequently, the presence of at least one of these three genes in a significant proportion of STEC suggests the potential of this vaccine to target a wide range of strains. The vaccination of pregnant sows led to an increase in specific IgG and IgA antibodies against the chimeric proteins, indicating successful immunization. Additionally, our results demonstrated the effective passive transfer of maternal antibodies to piglets, providing them with immediate, albeit temporary, humoral immunity against STEC. These humoral responses demonstrate the immunogenicity of the vaccine candidate and are preliminary indicators of its potential efficacy. However, further research is needed to conclusively evaluate its impact on STEC colonization and shedding. This study highlights the potential of maternal vaccination to protect piglets from ED and contributes to the development of vaccination strategies to reduce the prevalence of STEC in various animal reservoirs.
引用
收藏
页数:13
相关论文
共 50 条
  • [21] In silico analysis of Shiga toxins (Stxs) to identify new potential vaccine targets for Shiga toxin-producing Escherichia coli
    Maryam Golshani
    Mana Oloomi
    Saeid Bouzari
    In Silico Pharmacology, 5 (1)
  • [22] Role of Cellulose in Protecting Shiga Toxin-Producing Escherichia coli against Osmotic and Chlorine Stress
    Yoo, Byong K.
    Chen, Jinru
    JOURNAL OF FOOD PROTECTION, 2010, 73 (11) : 2084 - 2088
  • [23] A case of hemolytic uremic syndrome caused by Shiga toxin-producing Escherichia coli after pericardiectomy
    Tome, June
    Maselli, Daniel Barry
    Im, Roeun
    Amdahl, Matthew Brian
    Pfeifle, Daniel
    Hagen, Catherine
    Halland, Magnus
    CLINICAL JOURNAL OF GASTROENTEROLOGY, 2022, 15 (01) : 123 - 127
  • [24] Evaluation of synbiotics as gut health improvement agents against Shiga toxin-producing Escherichia coli isolated from the pig
    Kim, Bo-Ra
    Cho, Kyung Jin
    Kim, Doowan
    Cho, Jin Ho
    Lee, Jun Hyung
    Guevarra, Robin B.
    Lee, Sun Hee
    Kang, Jung Sun
    Cho, Won Tak
    Wattanaphansak, Suphot
    Kang, Bit Na
    Kim, Jong Nam
    Song, Minho
    Kim, Hyeun Bum
    JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY, 2019, 61 (02) : 55 - 60
  • [25] Evaluation of decontamination efficacy of commonly used antimicrobial interventions for beef carcasses against Shiga toxin-producing Escherichia coli
    Signorini, Marcelo
    Costa, Magdalena
    Teitelbaum, David
    Restovich, Viviana
    Brasesco, Hebe
    Garcia, Diego
    Superno, Valeria
    Petroli, Sandra
    Bruzzone, Mariana
    Arduini, Victor
    Vanzini, Monica
    Sucari, Adriana
    Suberbie, German
    Maricel, Turina
    Rodriguez, Ricardo
    Leotta, Gerardo A.
    MEAT SCIENCE, 2018, 142 : 44 - 51
  • [26] Preliminary evaluation of a procedure for improved detection of Shiga toxin-producing Escherichia coli in fecal specimens
    Hu, Jinxin
    Green, Donna
    Swoveland, Jennifer
    Grant, Michael
    Boyle, David S.
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2009, 65 (01) : 21 - 26
  • [27] Detection of Shiga toxin-producing Escherichia coli by PCR in cattle in Argentina Evaluation of two procedures
    Gioffré, A
    Meichtri, L
    Miliwebsky, E
    Baschkier, A
    Chillemi, G
    Romano, MI
    Estani, SS
    Cataldi, A
    Rodríguez, R
    Rivas, A
    VETERINARY MICROBIOLOGY, 2002, 87 (04) : 301 - 313
  • [28] A case of hemolytic uremic syndrome caused by Shiga toxin-producing Escherichia coli after pericardiectomy
    June Tome
    Daniel Barry Maselli
    Roeun Im
    Matthew Brian Amdahl
    Daniel Pfeifle
    Catherine Hagen
    Magnus Halland
    Clinical Journal of Gastroenterology, 2022, 15 : 123 - 127
  • [29] Evaluation of the SHIGA TOXIN QUIK CHEK after overnight enrichment as screening tool for Shiga toxin-producing Escherichia coli detection in human fecal samples
    Sanchez, Sergio
    Teresa Llorente, Maria
    Ramiro, Raquel
    Herrera-Leon, Laura
    Herrera-Leon, Silvia
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2019, 94 (03) : 218 - 222
  • [30] Evaluation of Antimicrobial Activity by Marine Nocardiopsis dassonvillei against Foodborne Listeria monocytogenes and Shiga Toxin-Producing Escherichia coli
    Ngema, Siyanda S.
    Khumalo, Solomuzi H.
    Ojo, Michael C.
    Pooe, Ofentse J.
    Malilehe, Tsolanku S.
    Basson, Albertus K.
    Madoroba, Evelyn
    MICROORGANISMS, 2023, 11 (10)