A Comparison between Hippurate Hydrolysis and Multiplex PCR for Differentiating Campylobacter coli and Campylobacter jejuni

被引：0
|
作者：
Adzitey, Frederick [1 ,2 ]
Corry, Janet [2 ]
机构：
[1] Univ Sains Malaysia, Sch Ind Technol, Usm Pulau Pinang 11800, Malaysia
[2] Univ Bristol, Dept Clin Vet Sci, Bristol BS40 5DU, Avon, England
来源：
TROPICAL LIFE SCIENCES RESEARCH | 2011年 / 22卷 / 01期
关键词：
C; coli; jejuni; Hippurate Hydrolysis; mPCR;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Species identification is important for epidemiological, clinical and treatment purposes. The aim of this study was to find out whether hippurate hydrolysis is a reliable test for differentiating between Campylobacter coli and Campylobacter jejuni. To achieve this, hippurate hydrolysis test was compared with multiplex Polymerase Chain Reaction (mPCR) for their ability to speciate C. coli and C. jejuni. Eighteen Campylobacter strains from poultry samples were used for this study. The results from 17 of the 18 strains were in agreement with both methods. Thus, the hippurate hydrolysis test can be used for distinguishing C. jejuni from C. coli although occasionally some strains of C. jejuni may be mis-identified as C. coli.
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页码：91 / 98
页数：8
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